Sleep deprivation by the 'flower pot' technique and spatial reference memory

Bradley D. Youngblood, Jun Zhou, Gennady N. Smagin, Donna H. Ryan, Ruth Babette Harris

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

This study investigated whether paradoxical, or rapid eye movement (REM), sleep deprivation (SD) affected spatial memory. SD was induced in male Wistar rats by housing them on small platforms over water. They fell into the water if they lost muscle tone. Controls were either housed in tanks with large platforms (TC) or in normal cages (CC). All rats had free access to food and water. Each day they were tested in a place-learning set task using a Morris water maze. The rats were released from 6 different starting points (sets) and allowed 2 min to find a submerged platform. Two trials were conducted from each starting point. SD caused a significant decrement in performance in Trial 1 from Day 2. By Day 4, when distance swum to find the platform was plotted against set, area under the curve was doubled in SD compared to that in TC and CC rats, indicating a significant impairment in reference spatial memory. There was no consistent effect on working memory, indicated by Trial 2. SD caused weight loss and increased serum corticosterone compared to that in CC rats. There were no differences in concentrations of hypothalamic, hippocampal, or cortical catecholamines or their metabolites. Serotonin metabolism was elevated in the hypothalamus and hippocampus in SD rats. These results indicate that SD induced in rats housed on small platforms causes a substantial impairment of reference memory. The memory deficit may not be specific to SD because the rats are physically stressed and lose some nonREM sleep when housed in these conditions.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalPhysiology and Behavior
Volume61
Issue number2
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Sleep Deprivation
Water
Spatial Memory
REM Sleep
Memory Disorders
Corticosterone
Short-Term Memory
Hypothalamus
Area Under Curve
Catecholamines
Wistar Rats
Weight Loss
Hippocampus
Serotonin
Sleep
Learning
Food
Muscles
Serum

Keywords

  • Catecholamines
  • Paradoxical sleep deprivation
  • Rats
  • Reference memory
  • Serotonin

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Sleep deprivation by the 'flower pot' technique and spatial reference memory. / Youngblood, Bradley D.; Zhou, Jun; Smagin, Gennady N.; Ryan, Donna H.; Harris, Ruth Babette.

In: Physiology and Behavior, Vol. 61, No. 2, 01.01.1997, p. 249-256.

Research output: Contribution to journalArticle

Youngblood, Bradley D. ; Zhou, Jun ; Smagin, Gennady N. ; Ryan, Donna H. ; Harris, Ruth Babette. / Sleep deprivation by the 'flower pot' technique and spatial reference memory. In: Physiology and Behavior. 1997 ; Vol. 61, No. 2. pp. 249-256.
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