Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation

Haitao Wu, Arnab Barik, Yisheng Lu, Chengyong Shen, Andrew Bowman, Lei Li, Anupama Sathyamurthy, Thiri W. Lin, Wen Cheng Xiong, Lin Mei

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuromuscular junction formation requires proper interaction between motoneurons and muscle cells. β-Catenin (Ctnnb1) in muscle is critical for motoneuron differentiation; however, little is known about the relevant retrograde signal. In this paper, we dissected which functions of muscle Ctnnb1 are critical by an in vivo transgenic approach. We show that Ctnnb1 mutant without the transactivation domain was unable to rescue presynaptic deficits of Ctnnb1 mutation, indicating the involvement of transcription regulation. On the other hand, the cell-adhesion function of Ctnnb1 is dispensable. We screened for proteins that may serve as a Ctnnb1-directed retrograde factor and identified Slit2 Transgenic expression of Slit2 specifically in the muscle was able to diminish presynaptic deficits by Ctnnb1 mutation in mice. Slit2 immobilized on beads was able to induce synaptophysin puncta in axons of spinal cord explants. Together, these observations suggest that Slit2 serves as a factor utilized by muscle Ctnnb1 to direct presynaptic differentiation.

Original languageEnglish (US)
Article numbere07266
Pages (from-to)1-20
Number of pages20
JournaleLife
Volume4
Issue numberJULY 2015
DOIs
StatePublished - Jun 10 2015

Fingerprint

Catenins
Muscle
Muscles
Motor Neurons
Synaptophysin
Mutation
Neuromuscular Junction
Cell Adhesion
Muscle Cells
Transcriptional Activation
Cell adhesion
Axons
Transcription
Spinal Cord
Cells
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Wu, H., Barik, A., Lu, Y., Shen, C., Bowman, A., Li, L., ... Mei, L. (2015). Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation. eLife, 4(JULY 2015), 1-20. [e07266]. https://doi.org/10.7554/eLife.07266

Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation. / Wu, Haitao; Barik, Arnab; Lu, Yisheng; Shen, Chengyong; Bowman, Andrew; Li, Lei; Sathyamurthy, Anupama; Lin, Thiri W.; Xiong, Wen Cheng; Mei, Lin.

In: eLife, Vol. 4, No. JULY 2015, e07266, 10.06.2015, p. 1-20.

Research output: Contribution to journalArticle

Wu, H, Barik, A, Lu, Y, Shen, C, Bowman, A, Li, L, Sathyamurthy, A, Lin, TW, Xiong, WC & Mei, L 2015, 'Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation', eLife, vol. 4, no. JULY 2015, e07266, pp. 1-20. https://doi.org/10.7554/eLife.07266
Wu H, Barik A, Lu Y, Shen C, Bowman A, Li L et al. Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation. eLife. 2015 Jun 10;4(JULY 2015):1-20. e07266. https://doi.org/10.7554/eLife.07266
Wu, Haitao ; Barik, Arnab ; Lu, Yisheng ; Shen, Chengyong ; Bowman, Andrew ; Li, Lei ; Sathyamurthy, Anupama ; Lin, Thiri W. ; Xiong, Wen Cheng ; Mei, Lin. / Slit2 as a β-catenin/ctnnb1-dependent retrograde signal for presynaptic differentiation. In: eLife. 2015 ; Vol. 4, No. JULY 2015. pp. 1-20.
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