Sm proteins specify germ cell fate by facilitating oskar mRNA localization

Graydon Bennett Gonsalvez, T. K. Rajendra, Ying Wen, Kavita Praveen, A. Gregory Matera

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Sm and Sm-like proteins are RNA-binding factors found in all three domains of life. Eukaryotic Sm proteins play essential roles in pre-mRNA splicing, forming the cores of spliceosomal small nuclear ribonucleoproteins (snRNPs). Recently, Sm proteins have been implicated in the specification of germ cells. However, a mechanistic understanding of their involvement in germline specification is lacking and a germline-specific RNA target has not been identified. We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in SmD3, and embryos from mutant mothers are defective in germline specification. We conclude that Sm proteins function to establish the germline in Drosophila, at least in part by mediating oskar mRNA localization.

Original languageEnglish (US)
Pages (from-to)2341-2351
Number of pages11
JournalDevelopment
Volume137
Issue number14
DOIs
StatePublished - Jul 15 2010

Fingerprint

Germ Cells
Messenger RNA
Drosophila
Small Nuclear Ribonucleoproteins
Proteins
RNA-Binding Proteins
RNA Precursors
Embryonic Structures
RNA
Mutation
messenger ribonucleoprotein

Keywords

  • Arginine methyltransferase
  • Dart5 (Capsuleen; PRMT5)
  • Drosophila
  • RNA localization
  • snRNP biogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Sm proteins specify germ cell fate by facilitating oskar mRNA localization. / Gonsalvez, Graydon Bennett; Rajendra, T. K.; Wen, Ying; Praveen, Kavita; Matera, A. Gregory.

In: Development, Vol. 137, No. 14, 15.07.2010, p. 2341-2351.

Research output: Contribution to journalArticle

Gonsalvez, GB, Rajendra, TK, Wen, Y, Praveen, K & Matera, AG 2010, 'Sm proteins specify germ cell fate by facilitating oskar mRNA localization', Development, vol. 137, no. 14, pp. 2341-2351. https://doi.org/10.1242/dev.042721
Gonsalvez, Graydon Bennett ; Rajendra, T. K. ; Wen, Ying ; Praveen, Kavita ; Matera, A. Gregory. / Sm proteins specify germ cell fate by facilitating oskar mRNA localization. In: Development. 2010 ; Vol. 137, No. 14. pp. 2341-2351.
@article{5f427379e8ce4cc2a16945aee1c1806d,
title = "Sm proteins specify germ cell fate by facilitating oskar mRNA localization",
abstract = "Sm and Sm-like proteins are RNA-binding factors found in all three domains of life. Eukaryotic Sm proteins play essential roles in pre-mRNA splicing, forming the cores of spliceosomal small nuclear ribonucleoproteins (snRNPs). Recently, Sm proteins have been implicated in the specification of germ cells. However, a mechanistic understanding of their involvement in germline specification is lacking and a germline-specific RNA target has not been identified. We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in SmD3, and embryos from mutant mothers are defective in germline specification. We conclude that Sm proteins function to establish the germline in Drosophila, at least in part by mediating oskar mRNA localization.",
keywords = "Arginine methyltransferase, Dart5 (Capsuleen; PRMT5), Drosophila, RNA localization, snRNP biogenesis",
author = "Gonsalvez, {Graydon Bennett} and Rajendra, {T. K.} and Ying Wen and Kavita Praveen and Matera, {A. Gregory}",
year = "2010",
month = "7",
day = "15",
doi = "10.1242/dev.042721",
language = "English (US)",
volume = "137",
pages = "2341--2351",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "14",

}

TY - JOUR

T1 - Sm proteins specify germ cell fate by facilitating oskar mRNA localization

AU - Gonsalvez, Graydon Bennett

AU - Rajendra, T. K.

AU - Wen, Ying

AU - Praveen, Kavita

AU - Matera, A. Gregory

PY - 2010/7/15

Y1 - 2010/7/15

N2 - Sm and Sm-like proteins are RNA-binding factors found in all three domains of life. Eukaryotic Sm proteins play essential roles in pre-mRNA splicing, forming the cores of spliceosomal small nuclear ribonucleoproteins (snRNPs). Recently, Sm proteins have been implicated in the specification of germ cells. However, a mechanistic understanding of their involvement in germline specification is lacking and a germline-specific RNA target has not been identified. We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in SmD3, and embryos from mutant mothers are defective in germline specification. We conclude that Sm proteins function to establish the germline in Drosophila, at least in part by mediating oskar mRNA localization.

AB - Sm and Sm-like proteins are RNA-binding factors found in all three domains of life. Eukaryotic Sm proteins play essential roles in pre-mRNA splicing, forming the cores of spliceosomal small nuclear ribonucleoproteins (snRNPs). Recently, Sm proteins have been implicated in the specification of germ cells. However, a mechanistic understanding of their involvement in germline specification is lacking and a germline-specific RNA target has not been identified. We demonstrate that Drosophila SmB and SmD3 are specific components of the oskar messenger ribonucleoprotein (mRNP), proper localization of which is required for establishing germline fate and embryonic patterning. Importantly, oskar mRNA is delocalized in females harboring a hypomorphic mutation in SmD3, and embryos from mutant mothers are defective in germline specification. We conclude that Sm proteins function to establish the germline in Drosophila, at least in part by mediating oskar mRNA localization.

KW - Arginine methyltransferase

KW - Dart5 (Capsuleen; PRMT5)

KW - Drosophila

KW - RNA localization

KW - snRNP biogenesis

UR - http://www.scopus.com/inward/record.url?scp=77954640418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954640418&partnerID=8YFLogxK

U2 - 10.1242/dev.042721

DO - 10.1242/dev.042721

M3 - Article

VL - 137

SP - 2341

EP - 2351

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 14

ER -