Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: A pilot study

Ricardo Santos Simões, José Maria Soares-Jr, Manuel J. Simões, Helena B. Nader, Maria Cândida P. Baracat, Gustavo Arantes R. Maciel, Paulo C. Serafini, Ricardo Azziz, Edmund C. Baracat

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Small leucine-rich proteoglycans (SLRPs) play an important role in tissue homeostasis and cell proliferation since these proteoglycans sequester multiple growth factors. However, the content of SLRPs in the endometrium of polycystic ovary syndrome (PCOS) women is unknown. Our purpose was to test the hypothesis that excessive endometrial proliferation in PCOS may be partly related to abnormalities in SLRPs. Methods: In a cross section study a total of 20 endometrial samples were collected from 10 patients with PCOS and 10 ovulatory women during their proliferative (pre-ovulatory) phase. The study subjects were matched for age, body mass index and race. The age range was 20 to 35 years. All volunteers were evaluated in reproductive endocrinology clinic, Gynecology Division, Clinics Hospital, University of São Paulo Medical School Profile and concentration of small leucine-rich proteoglycans (decorin, lumican, fibromodulin and biglycan) were determined by immunohistochemical testing and Western blotting. Results: Decorin and lumican demonstrated higher immunoreactivity and relative expression in the endometrium of women with PCOS compared to that of women with regular menstrual cycles. Conclusion: Our data suggests that the endometrium of PCOS women demonstrate a greater content of SLRP than controls; decorin and lumican, in particular, were found in higher concentrations in the endometrium of PCOS women during the proliferative phase. These differences may, in part, explain the excess of endometrial proliferation frequently observed in PCOS. Further studies are warranted.

Original languageEnglish (US)
Article number54
JournalJournal of Ovarian Research
Volume10
Issue number1
DOIs
StatePublished - Aug 8 2017

Fingerprint

Polycystic Ovary Syndrome
Endometrium
Decorin
Biglycan
Endocrinology
Proteoglycans
Menstrual Cycle
Small Leucine-Rich Proteoglycans
Medical Schools
Gynecology
Volunteers
Intercellular Signaling Peptides and Proteins
Body Mass Index
Homeostasis
Western Blotting
Cell Proliferation
Lumican

Keywords

  • Endometrium
  • Polycystic ovary syndrome
  • Proliferative phase
  • Small leucine-rich proteoglycans

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

Simões, R. S., Soares-Jr, J. M., Simões, M. J., Nader, H. B., Baracat, M. C. P., Maciel, G. A. R., ... Baracat, E. C. (2017). Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: A pilot study. Journal of Ovarian Research, 10(1), [54]. https://doi.org/10.1186/s13048-017-0349-9

Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women : A pilot study. / Simões, Ricardo Santos; Soares-Jr, José Maria; Simões, Manuel J.; Nader, Helena B.; Baracat, Maria Cândida P.; Maciel, Gustavo Arantes R.; Serafini, Paulo C.; Azziz, Ricardo; Baracat, Edmund C.

In: Journal of Ovarian Research, Vol. 10, No. 1, 54, 08.08.2017.

Research output: Contribution to journalArticle

Simões, RS, Soares-Jr, JM, Simões, MJ, Nader, HB, Baracat, MCP, Maciel, GAR, Serafini, PC, Azziz, R & Baracat, EC 2017, 'Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: A pilot study', Journal of Ovarian Research, vol. 10, no. 1, 54. https://doi.org/10.1186/s13048-017-0349-9
Simões, Ricardo Santos ; Soares-Jr, José Maria ; Simões, Manuel J. ; Nader, Helena B. ; Baracat, Maria Cândida P. ; Maciel, Gustavo Arantes R. ; Serafini, Paulo C. ; Azziz, Ricardo ; Baracat, Edmund C. / Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women : A pilot study. In: Journal of Ovarian Research. 2017 ; Vol. 10, No. 1.
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abstract = "Background: Small leucine-rich proteoglycans (SLRPs) play an important role in tissue homeostasis and cell proliferation since these proteoglycans sequester multiple growth factors. However, the content of SLRPs in the endometrium of polycystic ovary syndrome (PCOS) women is unknown. Our purpose was to test the hypothesis that excessive endometrial proliferation in PCOS may be partly related to abnormalities in SLRPs. Methods: In a cross section study a total of 20 endometrial samples were collected from 10 patients with PCOS and 10 ovulatory women during their proliferative (pre-ovulatory) phase. The study subjects were matched for age, body mass index and race. The age range was 20 to 35 years. All volunteers were evaluated in reproductive endocrinology clinic, Gynecology Division, Clinics Hospital, University of S{\~a}o Paulo Medical School Profile and concentration of small leucine-rich proteoglycans (decorin, lumican, fibromodulin and biglycan) were determined by immunohistochemical testing and Western blotting. Results: Decorin and lumican demonstrated higher immunoreactivity and relative expression in the endometrium of women with PCOS compared to that of women with regular menstrual cycles. Conclusion: Our data suggests that the endometrium of PCOS women demonstrate a greater content of SLRP than controls; decorin and lumican, in particular, were found in higher concentrations in the endometrium of PCOS women during the proliferative phase. These differences may, in part, explain the excess of endometrial proliferation frequently observed in PCOS. Further studies are warranted.",
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AU - Simões, Manuel J.

AU - Nader, Helena B.

AU - Baracat, Maria Cândida P.

AU - Maciel, Gustavo Arantes R.

AU - Serafini, Paulo C.

AU - Azziz, Ricardo

AU - Baracat, Edmund C.

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AB - Background: Small leucine-rich proteoglycans (SLRPs) play an important role in tissue homeostasis and cell proliferation since these proteoglycans sequester multiple growth factors. However, the content of SLRPs in the endometrium of polycystic ovary syndrome (PCOS) women is unknown. Our purpose was to test the hypothesis that excessive endometrial proliferation in PCOS may be partly related to abnormalities in SLRPs. Methods: In a cross section study a total of 20 endometrial samples were collected from 10 patients with PCOS and 10 ovulatory women during their proliferative (pre-ovulatory) phase. The study subjects were matched for age, body mass index and race. The age range was 20 to 35 years. All volunteers were evaluated in reproductive endocrinology clinic, Gynecology Division, Clinics Hospital, University of São Paulo Medical School Profile and concentration of small leucine-rich proteoglycans (decorin, lumican, fibromodulin and biglycan) were determined by immunohistochemical testing and Western blotting. Results: Decorin and lumican demonstrated higher immunoreactivity and relative expression in the endometrium of women with PCOS compared to that of women with regular menstrual cycles. Conclusion: Our data suggests that the endometrium of PCOS women demonstrate a greater content of SLRP than controls; decorin and lumican, in particular, were found in higher concentrations in the endometrium of PCOS women during the proliferative phase. These differences may, in part, explain the excess of endometrial proliferation frequently observed in PCOS. Further studies are warranted.

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