Solvation of dried dentin matrix by water and other polar solvents

David H. Pashley, Ricardo M. Carvalho, Franklin Chi Meng Tay, Kelli A. Agee, Kwang Won Lee

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Purpose: To develop a simple method for measuring the degree of solvation of dried, demineralized dentin matrix by water and other polar solvents. The null hypothesis was that there are no differences in expansion forces produced by different polar solvents. Materials and Methods: Midcoronal dentin discs were prepared from extracted, unerupted human third molars. The discs were cut into square specimens with surface areas of 2 × 2, 3 × 3 and 4 × 4 mm and thicknesses of 0.5, 1.0 and 1.5 mm. After demineralization in 0.5 M EDTA (pH 7), the dimensions of the specimens were measured both wet and dry. Dry specimens were held between two parallel steel plates connected to a 50 N load cell which measured the solvation force when water or other polar solvents were added. After measuring the expansion force induced by water, the specimens were fixed in glutaraldehyde and the trials repeated. On additional specimens, repeated measures of expansion forces were obtained using water, methanol, ethanol, n-propanol, n-butanol, ethylene glycol, formamide, hydroxyethylmethacrylate, N,N-dimethyl formamide and acetone in unfixed specimens. Results: Water produced hydration forces as high as 204 g before, and 428 g after glutaraldehyde treatment. The hydration force correlated better with specimen thickness than with surface area. Water solvated the matrix faster than methanol > ethanol > formamide > ethylene glycol. Hydroxyethylmethacrylate, N,N-dimethyl formamide and acetone were unable to solvate the dried matrix. Regression analysis of solvation force vs. Hansen's solubility parameters for dispersive, polar and hydrogen bonding forces demonstrated that solvation force correlations were highest with hydrogen bonding solubility parameters. Measurements of solvation forces provides a simple method for determining solvent-collagen matrix interactions.

Original languageEnglish (US)
Pages (from-to)97-102
Number of pages6
JournalAmerican Journal of Dentistry
Volume15
Issue number2
StatePublished - Apr 1 2002

Fingerprint

Dentin
Water
Dimethylformamide
Ethylene Glycol
Glutaral
Hydrogen Bonding
Acetone
Solubility
Methanol
Ethanol
1-Propanol
1-Butanol
Third Molar
Steel
Edetic Acid
Collagen
Regression Analysis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Pashley, D. H., Carvalho, R. M., Tay, F. C. M., Agee, K. A., & Lee, K. W. (2002). Solvation of dried dentin matrix by water and other polar solvents. American Journal of Dentistry, 15(2), 97-102.

Solvation of dried dentin matrix by water and other polar solvents. / Pashley, David H.; Carvalho, Ricardo M.; Tay, Franklin Chi Meng; Agee, Kelli A.; Lee, Kwang Won.

In: American Journal of Dentistry, Vol. 15, No. 2, 01.04.2002, p. 97-102.

Research output: Contribution to journalArticle

Pashley, DH, Carvalho, RM, Tay, FCM, Agee, KA & Lee, KW 2002, 'Solvation of dried dentin matrix by water and other polar solvents', American Journal of Dentistry, vol. 15, no. 2, pp. 97-102.
Pashley DH, Carvalho RM, Tay FCM, Agee KA, Lee KW. Solvation of dried dentin matrix by water and other polar solvents. American Journal of Dentistry. 2002 Apr 1;15(2):97-102.
Pashley, David H. ; Carvalho, Ricardo M. ; Tay, Franklin Chi Meng ; Agee, Kelli A. ; Lee, Kwang Won. / Solvation of dried dentin matrix by water and other polar solvents. In: American Journal of Dentistry. 2002 ; Vol. 15, No. 2. pp. 97-102.
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