Abstract
Trans-synaptic activation of gene expression is linked to long-term plastic adaptations in the nervous system. To examine the molecular program induced by synaptic activity, we have employed molecular cloning techniques to identify an immediate early gene that is rapidly induced in the brain. We here report the entire nucleotide sequence of the cDNA, which encodes an open reading frame of 396 amino acids. Within the hippocampus, constitutive expression was low. Basal levels of expression in the cortex were high but can be markedly reduced by blockade of N-methyl-D-aspartate receptors. By contrast, synaptic activity induced by convulsive seizures increased mRNA levels in neurons of the cortex and hippocampus. High-frequency stimulation of the perforant path resulted in long-term potentiation and a spatially confined dramatic increase in the level of mRNA in the granule cells of the ipsilateral dentate gyrus. Transcripts were localized to the soma and to the dendrites of the granule cells. The dendritic localization of the transcripts offers the potential for local synthesis of the protein at activated postsynaptic sites and may underlie synapse-specific modifications during long-term plastic events.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5734-5738 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 92 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jun 6 1995 |
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Keywords
- gene induction
- hippocampus
- long-term potentiation
- neuronal plasticity
- seizure
ASJC Scopus subject areas
- General
Cite this
Somatodendritic expression of an immediate early gene is regulated by synaptic activity. / Link, Wolfgang; Konietzko, Uwe; Kauselmann, Gunther; Krug, Manfred; Schwanke, Birgit; Frey, Julietta Uta; Kuhl, Dietmar.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 12, 06.06.1995, p. 5734-5738.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Somatodendritic expression of an immediate early gene is regulated by synaptic activity
AU - Link, Wolfgang
AU - Konietzko, Uwe
AU - Kauselmann, Gunther
AU - Krug, Manfred
AU - Schwanke, Birgit
AU - Frey, Julietta Uta
AU - Kuhl, Dietmar
PY - 1995/6/6
Y1 - 1995/6/6
N2 - Trans-synaptic activation of gene expression is linked to long-term plastic adaptations in the nervous system. To examine the molecular program induced by synaptic activity, we have employed molecular cloning techniques to identify an immediate early gene that is rapidly induced in the brain. We here report the entire nucleotide sequence of the cDNA, which encodes an open reading frame of 396 amino acids. Within the hippocampus, constitutive expression was low. Basal levels of expression in the cortex were high but can be markedly reduced by blockade of N-methyl-D-aspartate receptors. By contrast, synaptic activity induced by convulsive seizures increased mRNA levels in neurons of the cortex and hippocampus. High-frequency stimulation of the perforant path resulted in long-term potentiation and a spatially confined dramatic increase in the level of mRNA in the granule cells of the ipsilateral dentate gyrus. Transcripts were localized to the soma and to the dendrites of the granule cells. The dendritic localization of the transcripts offers the potential for local synthesis of the protein at activated postsynaptic sites and may underlie synapse-specific modifications during long-term plastic events.
AB - Trans-synaptic activation of gene expression is linked to long-term plastic adaptations in the nervous system. To examine the molecular program induced by synaptic activity, we have employed molecular cloning techniques to identify an immediate early gene that is rapidly induced in the brain. We here report the entire nucleotide sequence of the cDNA, which encodes an open reading frame of 396 amino acids. Within the hippocampus, constitutive expression was low. Basal levels of expression in the cortex were high but can be markedly reduced by blockade of N-methyl-D-aspartate receptors. By contrast, synaptic activity induced by convulsive seizures increased mRNA levels in neurons of the cortex and hippocampus. High-frequency stimulation of the perforant path resulted in long-term potentiation and a spatially confined dramatic increase in the level of mRNA in the granule cells of the ipsilateral dentate gyrus. Transcripts were localized to the soma and to the dendrites of the granule cells. The dendritic localization of the transcripts offers the potential for local synthesis of the protein at activated postsynaptic sites and may underlie synapse-specific modifications during long-term plastic events.
KW - gene induction
KW - hippocampus
KW - long-term potentiation
KW - neuronal plasticity
KW - seizure
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UR - http://www.scopus.com/inward/citedby.url?scp=0029046978&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.12.5734
DO - 10.1073/pnas.92.12.5734
M3 - Article
C2 - 7777577
AN - SCOPUS:0029046978
VL - 92
SP - 5734
EP - 5738
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -