Somatodendritic expression of an immediate early gene is regulated by synaptic activity

Wolfgang Link, Uwe Konietzko, Gunther Kauselmann, Manfred Krug, Birgit Schwanke, Julietta Uta Frey, Dietmar Kuhl

Research output: Contribution to journalArticle

496 Citations (Scopus)

Abstract

Trans-synaptic activation of gene expression is linked to long-term plastic adaptations in the nervous system. To examine the molecular program induced by synaptic activity, we have employed molecular cloning techniques to identify an immediate early gene that is rapidly induced in the brain. We here report the entire nucleotide sequence of the cDNA, which encodes an open reading frame of 396 amino acids. Within the hippocampus, constitutive expression was low. Basal levels of expression in the cortex were high but can be markedly reduced by blockade of N-methyl-D-aspartate receptors. By contrast, synaptic activity induced by convulsive seizures increased mRNA levels in neurons of the cortex and hippocampus. High-frequency stimulation of the perforant path resulted in long-term potentiation and a spatially confined dramatic increase in the level of mRNA in the granule cells of the ipsilateral dentate gyrus. Transcripts were localized to the soma and to the dendrites of the granule cells. The dendritic localization of the transcripts offers the potential for local synthesis of the protein at activated postsynaptic sites and may underlie synapse-specific modifications during long-term plastic events.

Original languageEnglish (US)
Pages (from-to)5734-5738
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number12
DOIs
StatePublished - Jun 6 1995

Fingerprint

Immediate-Early Genes
Plastics
Hippocampus
Perforant Pathway
Messenger RNA
Long-Term Potentiation
Dentate Gyrus
Molecular Cloning
Carisoprodol
Dendrites
N-Methyl-D-Aspartate Receptors
Synapses
Nervous System
Open Reading Frames
Seizures
Complementary DNA
Gene Expression
Neurons
Amino Acids
Brain

Keywords

  • gene induction
  • hippocampus
  • long-term potentiation
  • neuronal plasticity
  • seizure

ASJC Scopus subject areas

  • General

Cite this

Somatodendritic expression of an immediate early gene is regulated by synaptic activity. / Link, Wolfgang; Konietzko, Uwe; Kauselmann, Gunther; Krug, Manfred; Schwanke, Birgit; Frey, Julietta Uta; Kuhl, Dietmar.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 12, 06.06.1995, p. 5734-5738.

Research output: Contribution to journalArticle

Link, Wolfgang ; Konietzko, Uwe ; Kauselmann, Gunther ; Krug, Manfred ; Schwanke, Birgit ; Frey, Julietta Uta ; Kuhl, Dietmar. / Somatodendritic expression of an immediate early gene is regulated by synaptic activity. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 12. pp. 5734-5738.
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