Some properties of cardiac troponin T structure

N. B. Gusev, N. V. Barskaya, Alexander Dmitriyevich Verin, I. V. Duzhenkova, Z. A. Khuchua, A. O. Zheltova

Research output: Contribution to journalArticle

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Abstract

Troponin T is eluted in multiple peaks when the whole bovine cardiac troponin complex is subjected to DEAE-cellulose chromatography in the presence of 8 M-urea. The heterogeneity observed is due to the presence of two forms of troponin T, differing in their M(r) values, amino acid content, degree of phosphorylation and aggregation. Cardiac troponin T contains up to 0.8 mol of phosphate/mol of protein. Rabbit skeletal-muscle troponin T kinase phosphorylates the single site located in the N-terminal pentapeptide of cardiac troponin T. The composition of this peptide, (Ser,Asx,Glx,Glx)Val, is similar to that of skeletal-muscle troponin T. The single thiol group of cardiac troponin T is located some 50-70 residues from the N-terminus. The C-terminal sequence of cardiac troponin T is Trp-Lys, i.e. as is the case of skeletal-muscle troponin T.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalUnknown Journal
Volume213
Issue number1
DOIs
StatePublished - Jan 1 1983

Fingerprint

Troponin T
aggregation
Values
Group
Muscle
Skeletal Muscle
DEAE-Cellulose Chromatography
DEAE-Cellulose
Phosphorylation
Troponin
Chromatography
Sulfhydryl Compounds
Urea
Phosphotransferases
Agglomeration
Phosphates
Rabbits
Amino Acids
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gusev, N. B., Barskaya, N. V., Verin, A. D., Duzhenkova, I. V., Khuchua, Z. A., & Zheltova, A. O. (1983). Some properties of cardiac troponin T structure. Unknown Journal, 213(1), 123-129. https://doi.org/10.1042/bj2130123

Some properties of cardiac troponin T structure. / Gusev, N. B.; Barskaya, N. V.; Verin, Alexander Dmitriyevich; Duzhenkova, I. V.; Khuchua, Z. A.; Zheltova, A. O.

In: Unknown Journal, Vol. 213, No. 1, 01.01.1983, p. 123-129.

Research output: Contribution to journalArticle

Gusev, NB, Barskaya, NV, Verin, AD, Duzhenkova, IV, Khuchua, ZA & Zheltova, AO 1983, 'Some properties of cardiac troponin T structure', Unknown Journal, vol. 213, no. 1, pp. 123-129. https://doi.org/10.1042/bj2130123
Gusev NB, Barskaya NV, Verin AD, Duzhenkova IV, Khuchua ZA, Zheltova AO. Some properties of cardiac troponin T structure. Unknown Journal. 1983 Jan 1;213(1):123-129. https://doi.org/10.1042/bj2130123
Gusev, N. B. ; Barskaya, N. V. ; Verin, Alexander Dmitriyevich ; Duzhenkova, I. V. ; Khuchua, Z. A. ; Zheltova, A. O. / Some properties of cardiac troponin T structure. In: Unknown Journal. 1983 ; Vol. 213, No. 1. pp. 123-129.
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