Spatiotemporal expression of GM1 in murine medial pallial neural progenitor cells

Sean S. Liour, Michael B. Dinkins, Chen Ying Su, Robert K Yu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The expression of gangliosides is developmentally regulated in the central nervous system. The expression of GM1 in the neural progenitor cells of the telencephalonic ventricular zone (VZ) has been reported in several studies. However, information on the spatial and temporal regulation of GM1 expression in the VZ is still lacking. In this study, we characterized the expression of GM1 in the developing mouse telencephalon. At E13, GM1 is expressed in neuronal cells as well as in the VZ. The initial expression of GM1 in the VZ is restricted to regions close to the medial pallium. Fluorescence-activated cell sorting (FACS) analysis and characterization of E14 GM1-positive cells showed that they contain progenitor cells that proliferate in response to epidermal growth factor (EGF) and/or basic fibroblast growth factor (bFGF) stimulation. The results obtained from quantitative gene expression analysis of region-specific genes (Emx1, Lhx2, Ngn1, Ngn2, Pax6, Dlx2, Gsh2, Mash1, and Nkx2.1), using real-time polymerase chain reaction indicate that FACS of GM1-expressing cells in the fetal forebrain enriches for the medial pallial neural progenitor cells.

Original languageEnglish (US)
Pages (from-to)330-338
Number of pages9
JournalJournal of Comparative Neurology
Volume491
Issue number4
DOIs
StatePublished - Oct 31 2005

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Stem Cells
Flow Cytometry
Telencephalon
Gangliosides
Fibroblast Growth Factor 2
Prosencephalon
Epidermal Growth Factor
Real-Time Polymerase Chain Reaction
Central Nervous System
Gene Expression
Genes

Keywords

  • GM1
  • Medial pallium
  • Neural progenitor
  • Ventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spatiotemporal expression of GM1 in murine medial pallial neural progenitor cells. / Liour, Sean S.; Dinkins, Michael B.; Su, Chen Ying; Yu, Robert K.

In: Journal of Comparative Neurology, Vol. 491, No. 4, 31.10.2005, p. 330-338.

Research output: Contribution to journalArticle

Liour, Sean S. ; Dinkins, Michael B. ; Su, Chen Ying ; Yu, Robert K. / Spatiotemporal expression of GM1 in murine medial pallial neural progenitor cells. In: Journal of Comparative Neurology. 2005 ; Vol. 491, No. 4. pp. 330-338.
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