Species difference in the modulatory effect of κ agonist on 5-HT release from ground squirrel and rat hippocampus

Yan Cui; Tze fun Lee; Lawrence C.H. Wang

doi:10.1016/0304-3940(94)91095-2

Species difference in the modulatory effect of κ agonist on 5-HT release from ground squirrel and rat hippocampus

Yan Cui, Tze fun Lee, Lawrence C.H. Wang

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Inclusion of the κ agonist U50488 in the perifusion medium enhanced K⁺-stimulated 5-HT release from ground squirrel hippocampal slices, but reduced 5-HT outflow in both young and old rats' hippocampal slices. The stimulatory effect of U50488 on 5-HT release from ground squirrel hippocampus was not significantly attenuated by the non-specific opioid antagonist naloxone (10^-5 M), but was completely reversed by the specific κ antagonist nor-BNI (10^-6 M), and the voltage-dependent sodium channel blocker TTX (10^-6 M). In contrast, the inhibitory effect of U50488 on 5-HT release from the young rat hippocampus was attenuated by naloxone (10^-6 M), but not by nor-BNI (10^-6 M) or TTX (10^-6 M). These results illustrate a significant species difference in the modulatory effect of opioids on neurotransmitter release in the hippocampus.

Original language	English (US)
Pages (from-to)	126-128
Number of pages	3
Journal	Neuroscience Letters
Volume	175
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(94)91095-2
State	Published - Jul 4 1994
Externally published	Yes

Keywords

Hippocampus
Opioid
Serotonin release
U50488
κ Opioid receptor

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(94)91095-2

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title = "Species difference in the modulatory effect of κ agonist on 5-HT release from ground squirrel and rat hippocampus",

abstract = "Inclusion of the κ agonist U50488 in the perifusion medium enhanced K+-stimulated 5-HT release from ground squirrel hippocampal slices, but reduced 5-HT outflow in both young and old rats' hippocampal slices. The stimulatory effect of U50488 on 5-HT release from ground squirrel hippocampus was not significantly attenuated by the non-specific opioid antagonist naloxone (10-5 M), but was completely reversed by the specific κ antagonist nor-BNI (10-6 M), and the voltage-dependent sodium channel blocker TTX (10-6 M). In contrast, the inhibitory effect of U50488 on 5-HT release from the young rat hippocampus was attenuated by naloxone (10-6 M), but not by nor-BNI (10-6 M) or TTX (10-6 M). These results illustrate a significant species difference in the modulatory effect of opioids on neurotransmitter release in the hippocampus.",

keywords = "Hippocampus, Opioid, Serotonin release, U50488, κ Opioid receptor",

author = "Yan Cui and Lee, {Tze fun} and Wang, {Lawrence C.H.}",

note = "Funding Information: The present study was supported by a Natural Sciences and Engineering Research Council (Canada) grant (A6455) to L.W.",

year = "1994",

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doi = "10.1016/0304-3940(94)91095-2",

language = "English (US)",

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pages = "126--128",

journal = "Neuroscience Letters",

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T1 - Species difference in the modulatory effect of κ agonist on 5-HT release from ground squirrel and rat hippocampus

AU - Cui, Yan

AU - Lee, Tze fun

AU - Wang, Lawrence C.H.

N1 - Funding Information: The present study was supported by a Natural Sciences and Engineering Research Council (Canada) grant (A6455) to L.W.

PY - 1994/7/4

Y1 - 1994/7/4

N2 - Inclusion of the κ agonist U50488 in the perifusion medium enhanced K+-stimulated 5-HT release from ground squirrel hippocampal slices, but reduced 5-HT outflow in both young and old rats' hippocampal slices. The stimulatory effect of U50488 on 5-HT release from ground squirrel hippocampus was not significantly attenuated by the non-specific opioid antagonist naloxone (10-5 M), but was completely reversed by the specific κ antagonist nor-BNI (10-6 M), and the voltage-dependent sodium channel blocker TTX (10-6 M). In contrast, the inhibitory effect of U50488 on 5-HT release from the young rat hippocampus was attenuated by naloxone (10-6 M), but not by nor-BNI (10-6 M) or TTX (10-6 M). These results illustrate a significant species difference in the modulatory effect of opioids on neurotransmitter release in the hippocampus.

AB - Inclusion of the κ agonist U50488 in the perifusion medium enhanced K+-stimulated 5-HT release from ground squirrel hippocampal slices, but reduced 5-HT outflow in both young and old rats' hippocampal slices. The stimulatory effect of U50488 on 5-HT release from ground squirrel hippocampus was not significantly attenuated by the non-specific opioid antagonist naloxone (10-5 M), but was completely reversed by the specific κ antagonist nor-BNI (10-6 M), and the voltage-dependent sodium channel blocker TTX (10-6 M). In contrast, the inhibitory effect of U50488 on 5-HT release from the young rat hippocampus was attenuated by naloxone (10-6 M), but not by nor-BNI (10-6 M) or TTX (10-6 M). These results illustrate a significant species difference in the modulatory effect of opioids on neurotransmitter release in the hippocampus.

KW - Hippocampus

KW - Opioid

KW - Serotonin release

KW - U50488

KW - κ Opioid receptor

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SN - 0304-3940

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JO - Neuroscience Letters

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ER -

Species difference in the modulatory effect of κ agonist on 5-HT release from ground squirrel and rat hippocampus

Abstract

Keywords

ASJC Scopus subject areas

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