Specific IgG Subclass Antibody Levels and Phagocytosis of Serotype 14 Pneumococcus Following Immunization

A. St C. KANIUK, J. E. LORTAN, Michele Anne Monteil

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Complement and specific antibody directed against capsular polysaccharide are necessary for efficient phagocytosis of pneumococci. In normal adults, specific antibody to pneumococci is predominantly of the IgG2 subclass. However, the role of IgG2 in bacterial clearance is debatable. We therefore decided to investigate the relationship between specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus, before and after immunization with a pneumococcal capsular polysaccharide vaccine. Specific IgG subclass antibody was measured by an ELISA technique and the effect of serum on phagocytosis of radiolabelled pneumococci by normal polymorphs was determined. We found that in the presence of complement, phagocytosis correlated significantly with both specific IgGl and IgG2 antibody titres(r= 0.547, P= 0.002 and r= 0.464. P= 0.009, respectively). However, in decomplemented sera, the correlation with IgGl antibody was lost, whereas that with IgG2 antibody was strengthened (r= 0.641. P= < 0.001). The possibility that IgG2 binds to receptors on polymorphs should be considered.

Original languageEnglish (US)
Pages (from-to)96-98
Number of pages3
JournalScandinavian Journal of Immunology
Volume36
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Streptococcus pneumoniae
Phagocytosis
Immunization
Immunoglobulin G
Antibodies
Pneumococcal Vaccines
Serogroup
Serum
Polysaccharides
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology

Cite this

Specific IgG Subclass Antibody Levels and Phagocytosis of Serotype 14 Pneumococcus Following Immunization. / KANIUK, A. St C.; LORTAN, J. E.; Monteil, Michele Anne.

In: Scandinavian Journal of Immunology, Vol. 36, 01.01.1992, p. 96-98.

Research output: Contribution to journalArticle

KANIUK, A. St C. ; LORTAN, J. E. ; Monteil, Michele Anne. / Specific IgG Subclass Antibody Levels and Phagocytosis of Serotype 14 Pneumococcus Following Immunization. In: Scandinavian Journal of Immunology. 1992 ; Vol. 36. pp. 96-98.
@article{5964eb6b1e684cf98e0a6c35c795519f,
title = "Specific IgG Subclass Antibody Levels and Phagocytosis of Serotype 14 Pneumococcus Following Immunization",
abstract = "Complement and specific antibody directed against capsular polysaccharide are necessary for efficient phagocytosis of pneumococci. In normal adults, specific antibody to pneumococci is predominantly of the IgG2 subclass. However, the role of IgG2 in bacterial clearance is debatable. We therefore decided to investigate the relationship between specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus, before and after immunization with a pneumococcal capsular polysaccharide vaccine. Specific IgG subclass antibody was measured by an ELISA technique and the effect of serum on phagocytosis of radiolabelled pneumococci by normal polymorphs was determined. We found that in the presence of complement, phagocytosis correlated significantly with both specific IgGl and IgG2 antibody titres(r= 0.547, P= 0.002 and r= 0.464. P= 0.009, respectively). However, in decomplemented sera, the correlation with IgGl antibody was lost, whereas that with IgG2 antibody was strengthened (r= 0.641. P= < 0.001). The possibility that IgG2 binds to receptors on polymorphs should be considered.",
author = "KANIUK, {A. St C.} and LORTAN, {J. E.} and Monteil, {Michele Anne}",
year = "1992",
month = "1",
day = "1",
doi = "10.1111/j.1365-3083.1992.tb01628.x",
language = "English (US)",
volume = "36",
pages = "96--98",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Specific IgG Subclass Antibody Levels and Phagocytosis of Serotype 14 Pneumococcus Following Immunization

AU - KANIUK, A. St C.

AU - LORTAN, J. E.

AU - Monteil, Michele Anne

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Complement and specific antibody directed against capsular polysaccharide are necessary for efficient phagocytosis of pneumococci. In normal adults, specific antibody to pneumococci is predominantly of the IgG2 subclass. However, the role of IgG2 in bacterial clearance is debatable. We therefore decided to investigate the relationship between specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus, before and after immunization with a pneumococcal capsular polysaccharide vaccine. Specific IgG subclass antibody was measured by an ELISA technique and the effect of serum on phagocytosis of radiolabelled pneumococci by normal polymorphs was determined. We found that in the presence of complement, phagocytosis correlated significantly with both specific IgGl and IgG2 antibody titres(r= 0.547, P= 0.002 and r= 0.464. P= 0.009, respectively). However, in decomplemented sera, the correlation with IgGl antibody was lost, whereas that with IgG2 antibody was strengthened (r= 0.641. P= < 0.001). The possibility that IgG2 binds to receptors on polymorphs should be considered.

AB - Complement and specific antibody directed against capsular polysaccharide are necessary for efficient phagocytosis of pneumococci. In normal adults, specific antibody to pneumococci is predominantly of the IgG2 subclass. However, the role of IgG2 in bacterial clearance is debatable. We therefore decided to investigate the relationship between specific IgG subclass antibody levels and phagocytosis of serotype 14 pneumococcus, before and after immunization with a pneumococcal capsular polysaccharide vaccine. Specific IgG subclass antibody was measured by an ELISA technique and the effect of serum on phagocytosis of radiolabelled pneumococci by normal polymorphs was determined. We found that in the presence of complement, phagocytosis correlated significantly with both specific IgGl and IgG2 antibody titres(r= 0.547, P= 0.002 and r= 0.464. P= 0.009, respectively). However, in decomplemented sera, the correlation with IgGl antibody was lost, whereas that with IgG2 antibody was strengthened (r= 0.641. P= < 0.001). The possibility that IgG2 binds to receptors on polymorphs should be considered.

UR - http://www.scopus.com/inward/record.url?scp=0026640149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026640149&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.1992.tb01628.x

DO - 10.1111/j.1365-3083.1992.tb01628.x

M3 - Article

C2 - 1514059

AN - SCOPUS:0026640149

VL - 36

SP - 96

EP - 98

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

ER -