Specific microbiota-induced intestinal Th17 differentiation requires MHC class II but not GALT and mesenteric lymph nodes

Duke Geem, Oscar Medina-Contreras, Michelle McBride, Rodney D. Newberry, Pandelakis Koni, Timothy L. Denning

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

IL-17-expressing CD4+ T lymphocytes (Th17 cells) naturally reside in the intestine where specific cytokines and microbiota, such as segmented filamentous bacteria (SFB), promote their differentiation. Intestinal Th17 cells are thought to initially differentiate in the GALT and/or mesenteric lymph nodes upon Ag encounter and subsequently home to the lamina propria (LP) where they mediate effector functions. However, whether GALTand/or mesenteric lymph nodes are required for intestinal Th17 differentiation as well as how microbiota containing SFB regulate Ag-specific intestinal Th17 cells remain poorly defined. In this study, we observed that naive CD4+ T cells were abundant in the intestinal LP prior to weaning and that the accumulation of Th17 cells in response to microbiota containing SFB occurred in the absence of lymphotoxin-dependent lymphoid structures and the spleen. Furthermore, the differentiation of intestinal Th17 cells in the presence of microbiota containing SFB was dependent on MHC class II expression by CD11c+ cells. Lastly, the differentiation of Ag-specific Th17 cells required both the presence of cognate Ag and microbiota containing SFB. These findings suggest that microbiota containing SFB create an intestinal milieu that may induce Ag-specific Th17 differentiation against food and/or bacterial Ags directly in the intestinal LP.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalJournal of Immunology
Volume193
Issue number1
DOIs
StatePublished - Jul 1 2014

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Th17 Cells
Microbiota
Lymph Nodes
Bacteria
Mucous Membrane
T-Lymphocytes
Lymphotoxin-alpha
Interleukin-17
Weaning
Intestines
Gastrointestinal Microbiome
Spleen
Cytokines
Food

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Specific microbiota-induced intestinal Th17 differentiation requires MHC class II but not GALT and mesenteric lymph nodes. / Geem, Duke; Medina-Contreras, Oscar; McBride, Michelle; Newberry, Rodney D.; Koni, Pandelakis; Denning, Timothy L.

In: Journal of Immunology, Vol. 193, No. 1, 01.07.2014, p. 431-438.

Research output: Contribution to journalArticle

Geem, Duke ; Medina-Contreras, Oscar ; McBride, Michelle ; Newberry, Rodney D. ; Koni, Pandelakis ; Denning, Timothy L. / Specific microbiota-induced intestinal Th17 differentiation requires MHC class II but not GALT and mesenteric lymph nodes. In: Journal of Immunology. 2014 ; Vol. 193, No. 1. pp. 431-438.
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