Splenic CD21high IGMhigh (MZ) B cells can quickly generate into plasma cells after in-vivo T-independent antigen stimulation

Alyce M. Oliver, F. Martin, J. F. Kearney

Research output: Contribution to journalArticle

Abstract

We have previously shown that CO21high IgMhigh mouse B cells enriched in the marginal zone (MZ) are unique in both phenotype and function compared to CD21int. IgMint. follicular (FO) B cells. In the VH81x transgenic mouse, a predominant heavy and light chain pair gives rise to a prevailing idiotype (Id) which is expressed in the majority of B cells in the MZ. These Id+ B cells function similarly to MZ B cells found in other normal mouse strains. Since Id+ B cells bind phosphorylcholine (PC), we investigated the response to a T-independent antigen from a heat killed Strep. pneumoniae vaccine in these transgenic mice. 24 hours following i.v. injection of the vaccine, syndecanhigh, cytoplasmic μhigh, CD38low, B220low plasma cells were observed in the red pulp and this response peaked at 3 days. These plasma cells were derived from MZ B cells as shown by expression of the predominant Id found in the VH81x MZ B cell population. Furthermore, the MZ B cells incorporated more BrdU than FO B cells 3 days after immunization, indicating that these cells were undergoing more rapid cellular division. Finally, serum Ig levels indicated that Id+ Ig was secreted following immunization. Thus MZ B cells are able to quickly respond to T-independent antigens by differentiating into plasma cells and secreting antigen specific Ig.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

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T Independent Antigens
plasma cells
Plasma Cells
B-lymphocytes
B-Lymphocytes
Cells
antigens
Plasmas
Immunization
mice
Transgenic Mice
immunization
Vaccines
genetically modified organisms
vaccines
Phosphorylcholine
Bromodeoxyuridine
pneumonia
Pulp
pulp

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Splenic CD21high IGMhigh (MZ) B cells can quickly generate into plasma cells after in-vivo T-independent antigen stimulation. / Oliver, Alyce M.; Martin, F.; Kearney, J. F.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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