Squamous carcinoma cells influence monocyte phenotype and suppress lipopolysaccharide-induced tnf-alpha in monocytes

Aroonwan Lam-ubol, Dustin Hopkin, Elena M. Letuchy, Zoya Bronislavovna Kurago

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bacteria and chronic inflammation are present in squamous cell carcinoma of the head and neck (HNSCC), but their roles in the pathogenesis of HNSCC are unclear. Our studies described here revealed that human monocytes co-cultured short term with HNSCC cells were more likely to express CD16, and CD16 + small mononuclear cells were common in HNSCC specimens. In addition, we identified monocytes as the primary source of LPS-induced IL-6 and TNF-alpha in the monocyte-HNSCC co-cultures. Remarkably, relative to LPS-stimulated monocytes cultured alone, HNSCC cells profoundly suppressed LPS-induced TNF-alpha in monocytes, without compromising IL-6 production. High levels of cytoprotective factors like IL-6 and low levels of TNF-alpha are important for the tumor microenvironment that enables tumor cell survival, affects monocyte differentiation and may contribute to tumor colonization by bacteria. This study provides novel observations that HNSCC cells affect monocyte phenotype and function, which are relevant to the regulation of the HNSCC microenvironment.

Original languageEnglish (US)
Pages (from-to)207-223
Number of pages17
JournalInflammation
Volume33
Issue number4
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

Fingerprint

Lipopolysaccharides
Monocytes
Squamous Cell Carcinoma
Phenotype
Interleukin-6
Tumor Necrosis Factor-alpha
Bacteria
Tumor Microenvironment
Coculture Techniques
Neoplasms
Cell Survival
Inflammation

Keywords

  • Carcinoma
  • Inflammation
  • Lipopolysaccharide
  • Monocytes
  • TNF-alpha
  • Tumor microenvironment

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Squamous carcinoma cells influence monocyte phenotype and suppress lipopolysaccharide-induced tnf-alpha in monocytes. / Lam-ubol, Aroonwan; Hopkin, Dustin; Letuchy, Elena M.; Kurago, Zoya Bronislavovna.

In: Inflammation, Vol. 33, No. 4, 01.08.2010, p. 207-223.

Research output: Contribution to journalArticle

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