Stability, delivery and functions of human sperm RNAs at fertilization

Edward Sendler, Graham D. Johnson, Shihong Mao, Robert J. Goodrich, Michael Peter Diamond, Russ Hauser, Stephen A. Krawetz

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Increasing attention has focused on the significance of RNA in sperm, in light of its contribution to the birth and long-term health of a child, role in sperm function and diagnostic potential. As the composition of sperm RNA is in flux, assigning specific roles to individual RNAs presents a significant challenge. For the first time RNA-seq was used to characterize the population of coding and non-coding transcripts in human sperm. Examining RNA representation as a function of multiple methods of library preparation revealed unique features indicative of very specific and stage-dependent maturation and regulation of sperm RNA, illuminating their various transitional roles. Correlation of sperm transcript abundance with epigenetic marks suggested roles for these elements in the pre-and post-fertilization genome. Several classes of non-coding RNAs including lncRNAs, CARs, pri-miRNAs, novel elements and mRNAs have been identified which, based on factors including relative abundance, integrity in sperm, available knockout data of embryonic effect and presence or absence in the unfertilized human oocyte, are likely to be essential male factors critical to early post-fertilization development. The diverse and unique attributes of sperm transcripts that were revealed provides the first detailed analysis of the biology and anticipated clinical significance of spermatozoal RNAs.

Original languageEnglish (US)
Pages (from-to)4104-4117
Number of pages14
JournalNucleic Acids Research
Volume41
Issue number7
DOIs
StatePublished - Apr 1 2013
Externally publishedYes

Fingerprint

Fertilization
Spermatozoa
RNA
Long Noncoding RNA
Sperm Maturation
Untranslated RNA
MicroRNAs
Epigenomics
Libraries
Oocytes
Parturition
Genome
Messenger RNA
Population

ASJC Scopus subject areas

  • Genetics

Cite this

Sendler, E., Johnson, G. D., Mao, S., Goodrich, R. J., Diamond, M. P., Hauser, R., & Krawetz, S. A. (2013). Stability, delivery and functions of human sperm RNAs at fertilization. Nucleic Acids Research, 41(7), 4104-4117. https://doi.org/10.1093/nar/gkt132

Stability, delivery and functions of human sperm RNAs at fertilization. / Sendler, Edward; Johnson, Graham D.; Mao, Shihong; Goodrich, Robert J.; Diamond, Michael Peter; Hauser, Russ; Krawetz, Stephen A.

In: Nucleic Acids Research, Vol. 41, No. 7, 01.04.2013, p. 4104-4117.

Research output: Contribution to journalArticle

Sendler, E, Johnson, GD, Mao, S, Goodrich, RJ, Diamond, MP, Hauser, R & Krawetz, SA 2013, 'Stability, delivery and functions of human sperm RNAs at fertilization', Nucleic Acids Research, vol. 41, no. 7, pp. 4104-4117. https://doi.org/10.1093/nar/gkt132
Sendler, Edward ; Johnson, Graham D. ; Mao, Shihong ; Goodrich, Robert J. ; Diamond, Michael Peter ; Hauser, Russ ; Krawetz, Stephen A. / Stability, delivery and functions of human sperm RNAs at fertilization. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 7. pp. 4104-4117.
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