Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions

Yingchuan Li, Xiaoqing Pan, Michelle L. Roberts, Pengyuan Liu, Theodore A. Kotchen, Allen W. Cowley, David L. Mattson, Yong Liu, Mingyu Liang, Srividya Kidambi

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Aim: To test whether DNA samples stored for a prolonged period (20 years) under various storage conditions could be used for comparative methylation studies using reduced representation bisulfite sequencing. Patients & methods: Five groups of human blood DNA samples (n = 5-6/group) were compared. The groupings were based on the anticoagulant used and storage temperature and duration. Results: Methylation profiles of defined genomic regions in the DNA or blood samples archived for 20 years were similar across all storage temperatures, including 4°C. The level of intersample similarity in archived samples was not significantly different than that in recently collected samples. Conclusion: Archived samples, including DNA stored at 4°C for 20 years, are suitable for comparative studies of DNA methylation.

Original languageEnglish (US)
Pages (from-to)797-811
Number of pages15
JournalEpigenomics
Volume10
Issue number6
DOIs
StatePublished - Jun 2018
Externally publishedYes

Keywords

  • methylation profiles
  • stability
  • storage conditions

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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    Li, Y., Pan, X., Roberts, M. L., Liu, P., Kotchen, T. A., Cowley, A. W., Mattson, D. L., Liu, Y., Liang, M., & Kidambi, S. (2018). Stability of global methylation profiles of whole blood and extracted DNA under different storage durations and conditions. Epigenomics, 10(6), 797-811. https://doi.org/10.2217/epi-2018-0025