I-(rLH) and 125I-(rFSH) were dissolved in sample buffer containing 0-0.1 % (SDS) and subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions at room temperature. 125I-rFSH migrated as a single band, while 125I-rLH migrated as two bands corresponding to rLH and rLH subunits suggesting partial dissociation. The dissociation of rLH increased as the concentration of SDS in the sample buffer was increased. However, electrophoresis of 125I-rLH at 4°C under nonreducing conditions caused minimal dissociation. These data suggest that (1) substantial dissociation of rLH, but not rFSH, occurs during nonreducing SDS-PAGE at room temperature and (2) running the gels at 4°C minimizes the dissociation of rLH.
ASJC Scopus subject areas
- Reproductive Medicine