Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines

Yupa Phonphok, Ken S. Rosenthal

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25°C or 37°C caused the release clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride) (10 mM), chloroquin (0.1 mM) and monodansyleadaverine (10 mM) significantly reduced the extent of dissociation.

Original languageEnglish (US)
Pages (from-to)188-190
Number of pages3
JournalFEBS Letters
Volume281
Issue number1-2
DOIs
StatePublished - Apr 9 1991
Externally publishedYes

Keywords

  • Amantadine
  • Clathrin
  • Clathrin coated vesicle
  • Hydrophobic amine
  • Tromantadine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines'. Together they form a unique fingerprint.

  • Cite this