Stabilized β-catenin potentiates Fas-mediated T cell apoptosis

Zhaofeng Huang, Ruiqing Wang, Huimin Xie, Weirong Shang, Santhakumar Manicassamy, Zuoming Sun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In response to Ag stimulation, Ag-specific T cells proliferate and accumulate in the peripheral lymphoid tissues. To avoid excessive T cell accumulation, the immune system has developed mechanisms to delete clonally expanded T cells. Fas/FasL-mediated apoptosis plays a critical role in the deletion of activated peripheral T cells, which is clearly demonstrated by superantigen (staphylococcal enterotoxin B)-induced deletion of Vβ8 + T cells. Using transgcnic mice expressing a stabilized β-catenin (β-cat Tg), we show here that β-catenin was able to enhance apoptosis of activated T cells by up-regulating Fas. In response to staphylococcal enterotoxin B stimulation, β-cat Tg mice exhibited accelerated deletion of CD4 +Vβ8 + T cells compared with wild type mice. Surface Fas levels were significantly higher on activated T cells obtained from β-cat Tg mice than that from wild type mice. Additionally, T cells from β-cat Tg mice w ere more sensitive to apoptosis induced by crosslinking Fas, activation-induced cell death, and to apoptosis induced by cytokine withdrawal. Lastly, β-catenin bound to and stimulated the Fas promoter. Therefore, our data demonstrated that the β-catenin pathway was able to promote the apoptosis of activated T cells in part via up-regulation of Fas.

Original languageEnglish (US)
Pages (from-to)6586-6592
Number of pages7
JournalJournal of Immunology
Volume180
Issue number10
StatePublished - Dec 1 2008
Externally publishedYes

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Catenins
Apoptosis
T-Lymphocytes
Cats
Superantigens
Lymphoid Tissue
Immune System
Cell Death
Up-Regulation
Cytokines

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Huang, Z., Wang, R., Xie, H., Shang, W., Manicassamy, S., & Sun, Z. (2008). Stabilized β-catenin potentiates Fas-mediated T cell apoptosis. Journal of Immunology, 180(10), 6586-6592.

Stabilized β-catenin potentiates Fas-mediated T cell apoptosis. / Huang, Zhaofeng; Wang, Ruiqing; Xie, Huimin; Shang, Weirong; Manicassamy, Santhakumar; Sun, Zuoming.

In: Journal of Immunology, Vol. 180, No. 10, 01.12.2008, p. 6586-6592.

Research output: Contribution to journalArticle

Huang, Z, Wang, R, Xie, H, Shang, W, Manicassamy, S & Sun, Z 2008, 'Stabilized β-catenin potentiates Fas-mediated T cell apoptosis', Journal of Immunology, vol. 180, no. 10, pp. 6586-6592.
Huang Z, Wang R, Xie H, Shang W, Manicassamy S, Sun Z. Stabilized β-catenin potentiates Fas-mediated T cell apoptosis. Journal of Immunology. 2008 Dec 1;180(10):6586-6592.
Huang, Zhaofeng ; Wang, Ruiqing ; Xie, Huimin ; Shang, Weirong ; Manicassamy, Santhakumar ; Sun, Zuoming. / Stabilized β-catenin potentiates Fas-mediated T cell apoptosis. In: Journal of Immunology. 2008 ; Vol. 180, No. 10. pp. 6586-6592.
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