Steady-state detection of cholesterol contained in the plasma membrane of a single cell using lipid bilayer-modified microelectrodes incorporating cholesterol oxidase

Anando Devadoss, James Burgess

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Platinum microelectrodes modified with a lipid bilayer membrane incorporating cholesterol oxidase are used for detection of cholesterol contained in the plasma membrane of a single cell. Amperometric responses are consistent with enzymatic catalysis being rate limiting and cholesterol diffusing laterally in the plasma membrane to the electrode contact site. Importantly, electrode response appears to correlate with the cholesterol content of the cell plasma membrane. The electrodes should be useful for characterizing cellular cholesterol tracking pathways involved in pathogenesis of disease.

Original languageEnglish (US)
Pages (from-to)10214-10215
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number33
DOIs
StatePublished - Aug 25 2004

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Cholesterol Oxidase
Lipid bilayers
Microelectrodes
Cholesterol
Lipid Bilayers
Cell membranes
Cell Membrane
Electrodes
Membrane Lipids
Platinum
Catalysis
Membranes
Oxidoreductases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Steady-state detection of cholesterol contained in the plasma membrane of a single cell using lipid bilayer-modified microelectrodes incorporating cholesterol oxidase. / Devadoss, Anando; Burgess, James.

In: Journal of the American Chemical Society, Vol. 126, No. 33, 25.08.2004, p. 10214-10215.

Research output: Contribution to journalArticle

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