Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms

Michael L. Whitfield, Lian Xing Zheng, Amy Baldwin, Tomohiko Ohta, Myra M. Hurt, William F. Marzluff

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

The expression of the replication-dependent histone mRNAs is tightly regulated during the cell cycle. As cells progress from G1 to S phase, histone mRNA levels increase 35-fold, and they decrease again during G2 phase. Replication-dependent histone mRNAs are the only metazoan mRNAs that lack polyadenylated tails, ending instead in a conserved stem-loop. Much of the cell cycle regulation is posttranscriptional and is mediated by the 3' stem-loop. A 31-kDa stem-loop binding protein (SLBP) binds the 3' end of histone mRNA. The SLBP is necessary for pre-mRNA processing and accompanies the histone mRNA to the cytoplasm, where it is a component of the histone messenger RNP. We used synchronous CHO cells selected by mitotic shakeoff and HeLa cells synchronized at the G1/S or the M/G1 boundary to study the regulation of SLBP during the cell cycle. In each system the amount of SLBP is regulated during the cell cycle, increasing 10- to 20-fold in the late G1 and then decreasing in the S/G2 border. SLBP mRNA levels are constant during the cell cycle. SLBP is regulated at the level of translation as cells progress from G1 to S phase, and the protein is rapidly degraded as they progress into G2. Regulation of SLBP may account for the posttranscriptional component of the cell cycle regulation of histone mRNA.

Original languageEnglish (US)
Pages (from-to)4188-4198
Number of pages11
JournalMolecular and Cellular Biology
Volume20
Issue number12
DOIs
StatePublished - Jun 1 2000

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Histones
Cell Cycle
Carrier Proteins
Messenger RNA
Proteins
S Phase
CHO Cells
G2 Phase
RNA Precursors
HeLa Cells
Cytoplasm

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms. / Whitfield, Michael L.; Zheng, Lian Xing; Baldwin, Amy; Ohta, Tomohiko; Hurt, Myra M.; Marzluff, William F.

In: Molecular and Cellular Biology, Vol. 20, No. 12, 01.06.2000, p. 4188-4198.

Research output: Contribution to journalArticle

Whitfield, Michael L. ; Zheng, Lian Xing ; Baldwin, Amy ; Ohta, Tomohiko ; Hurt, Myra M. ; Marzluff, William F. / Stem-loop binding protein, the protein that binds the 3' end of histone mRNA, is cell cycle regulated by both translational and posttranslational mechanisms. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 12. pp. 4188-4198.
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