Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14

Han Fei Ding, Sylvie Rimsky, Susan C. Batson, Michael Bustin, Ulla Hansen

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

The high-mobility group protein 14 (HMG-14) is a non-histone chromosomal protein that is preferentially associated with transcriptionally active chromatin. To assess the effect of HMG-14 on transcription by RNA polymerase II, in vivo-assembled chromatin with elevated amounts of HMG-14 was obtained. Here it is shown that HMG-14 enhanced transcription on chromatin templates but not on DNA templates. This protein stimulated the rate of elongation by RNA polymerase II but not the level of initiation of transcription. These findings suggest that the association of HMG-14 with nucleosomes is part of the cellular process involved in the generation of transcriptionally active chromatin.

Original languageEnglish (US)
Pages (from-to)796-799
Number of pages4
JournalScience
Volume265
Issue number5173
DOIs
StatePublished - Aug 5 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

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