Strain-dependent stimulation of growth in leptin-treated obese db/db mice

Abram M. Madiehe, Sadie Hebert, Tiffany D. Mitchell, Ruth Babette Harris

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Leptin increases the proliferation of various cell types in vitro, and we reported that background strain influences the metabolic responses to leptin in db/db mice, which express short-form, but not long-form, leptin receptors. Here, we examined the effects of leptin on growth of young C57BL/Ks, C57BL/6J, and C57BL/3J db/db mice. Intraperitoneal infusions of 20 μg leptin/d for 26 d increased the food intake of C57BL/6J mice by 15% (P < 0.01), but had no effect in C57BL/Ks db/db mice. Leptin-infused C57BL/6J db/db mice gained more weight (∼20%; P < 0.04) than PBS-infused controls. The increased weight was sustained after leptin infusion ended. Leptin had no effect on weight gain or food intake of C57BL/3J db/db mice, which only express the soluble leptin receptor. A single leptin injection increased MAPK phosphorylation in liver by 40% (P < 0.001) and that in muscle tissues by 20% (P < 0.001) in C57BL/6J mice, but did not change phosphorylation in C57BL/3J db/db mice. These results suggest that leptin increases the weight gain of C57BL/6J db/db mice by activating the MAPK pathway through a mechanism that is dependent on short-form leptin receptors. This response may be masked by activation of the long-form receptor in wild-type animals that lose body fat during leptin treatment.

Original languageEnglish (US)
Pages (from-to)3875-3883
Number of pages9
JournalEndocrinology
Volume143
Issue number10
DOIs
StatePublished - Oct 1 2002

Fingerprint

Leptin
Growth
Leptin Receptors
Inbred C57BL Mouse
Weight Gain
Eating
Phosphorylation
Parenteral Infusions
Weights and Measures
Wild Animals
Adipose Tissue
Cell Proliferation
Muscles
Injections
Liver

ASJC Scopus subject areas

  • Endocrinology

Cite this

Strain-dependent stimulation of growth in leptin-treated obese db/db mice. / Madiehe, Abram M.; Hebert, Sadie; Mitchell, Tiffany D.; Harris, Ruth Babette.

In: Endocrinology, Vol. 143, No. 10, 01.10.2002, p. 3875-3883.

Research output: Contribution to journalArticle

Madiehe, Abram M. ; Hebert, Sadie ; Mitchell, Tiffany D. ; Harris, Ruth Babette. / Strain-dependent stimulation of growth in leptin-treated obese db/db mice. In: Endocrinology. 2002 ; Vol. 143, No. 10. pp. 3875-3883.
@article{beaa27098ac444e4b867a7d90f572fe7,
title = "Strain-dependent stimulation of growth in leptin-treated obese db/db mice",
abstract = "Leptin increases the proliferation of various cell types in vitro, and we reported that background strain influences the metabolic responses to leptin in db/db mice, which express short-form, but not long-form, leptin receptors. Here, we examined the effects of leptin on growth of young C57BL/Ks, C57BL/6J, and C57BL/3J db/db mice. Intraperitoneal infusions of 20 μg leptin/d for 26 d increased the food intake of C57BL/6J mice by 15{\%} (P < 0.01), but had no effect in C57BL/Ks db/db mice. Leptin-infused C57BL/6J db/db mice gained more weight (∼20{\%}; P < 0.04) than PBS-infused controls. The increased weight was sustained after leptin infusion ended. Leptin had no effect on weight gain or food intake of C57BL/3J db/db mice, which only express the soluble leptin receptor. A single leptin injection increased MAPK phosphorylation in liver by 40{\%} (P < 0.001) and that in muscle tissues by 20{\%} (P < 0.001) in C57BL/6J mice, but did not change phosphorylation in C57BL/3J db/db mice. These results suggest that leptin increases the weight gain of C57BL/6J db/db mice by activating the MAPK pathway through a mechanism that is dependent on short-form leptin receptors. This response may be masked by activation of the long-form receptor in wild-type animals that lose body fat during leptin treatment.",
author = "Madiehe, {Abram M.} and Sadie Hebert and Mitchell, {Tiffany D.} and Harris, {Ruth Babette}",
year = "2002",
month = "10",
day = "1",
doi = "10.1210/en.2002-220362",
language = "English (US)",
volume = "143",
pages = "3875--3883",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "10",

}

TY - JOUR

T1 - Strain-dependent stimulation of growth in leptin-treated obese db/db mice

AU - Madiehe, Abram M.

AU - Hebert, Sadie

AU - Mitchell, Tiffany D.

AU - Harris, Ruth Babette

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Leptin increases the proliferation of various cell types in vitro, and we reported that background strain influences the metabolic responses to leptin in db/db mice, which express short-form, but not long-form, leptin receptors. Here, we examined the effects of leptin on growth of young C57BL/Ks, C57BL/6J, and C57BL/3J db/db mice. Intraperitoneal infusions of 20 μg leptin/d for 26 d increased the food intake of C57BL/6J mice by 15% (P < 0.01), but had no effect in C57BL/Ks db/db mice. Leptin-infused C57BL/6J db/db mice gained more weight (∼20%; P < 0.04) than PBS-infused controls. The increased weight was sustained after leptin infusion ended. Leptin had no effect on weight gain or food intake of C57BL/3J db/db mice, which only express the soluble leptin receptor. A single leptin injection increased MAPK phosphorylation in liver by 40% (P < 0.001) and that in muscle tissues by 20% (P < 0.001) in C57BL/6J mice, but did not change phosphorylation in C57BL/3J db/db mice. These results suggest that leptin increases the weight gain of C57BL/6J db/db mice by activating the MAPK pathway through a mechanism that is dependent on short-form leptin receptors. This response may be masked by activation of the long-form receptor in wild-type animals that lose body fat during leptin treatment.

AB - Leptin increases the proliferation of various cell types in vitro, and we reported that background strain influences the metabolic responses to leptin in db/db mice, which express short-form, but not long-form, leptin receptors. Here, we examined the effects of leptin on growth of young C57BL/Ks, C57BL/6J, and C57BL/3J db/db mice. Intraperitoneal infusions of 20 μg leptin/d for 26 d increased the food intake of C57BL/6J mice by 15% (P < 0.01), but had no effect in C57BL/Ks db/db mice. Leptin-infused C57BL/6J db/db mice gained more weight (∼20%; P < 0.04) than PBS-infused controls. The increased weight was sustained after leptin infusion ended. Leptin had no effect on weight gain or food intake of C57BL/3J db/db mice, which only express the soluble leptin receptor. A single leptin injection increased MAPK phosphorylation in liver by 40% (P < 0.001) and that in muscle tissues by 20% (P < 0.001) in C57BL/6J mice, but did not change phosphorylation in C57BL/3J db/db mice. These results suggest that leptin increases the weight gain of C57BL/6J db/db mice by activating the MAPK pathway through a mechanism that is dependent on short-form leptin receptors. This response may be masked by activation of the long-form receptor in wild-type animals that lose body fat during leptin treatment.

UR - http://www.scopus.com/inward/record.url?scp=0036772358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036772358&partnerID=8YFLogxK

U2 - 10.1210/en.2002-220362

DO - 10.1210/en.2002-220362

M3 - Article

C2 - 12239099

AN - SCOPUS:0036772358

VL - 143

SP - 3875

EP - 3883

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 10

ER -