Structural Analysis of a Glycolipid Head Group with One- and Two-State NMR Pseudoenergy Approaches

J. N. Scarsdale, J. H. Prestegard, Robert K Yu

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The solution conformation of the oligosaccharide head group of the glycolipid globoside has been determined with the use of 1 H two-dimensional NMR methods. The intensities of cross peaks in cross relaxation correlated experiments have been analyzed in terms of interproton distances, and the distances have been incorporated as a pseudoenergy in a conformational energy calculation. Minimum energy conformations have been determined by using both one- and two-state models. Conformations are consistent with a previously proposed “L” shape for the molecule. Comparison of the results from the two models suggests the possibility of conformational flexibility to exist for the terminal residue of the head group.

Original languageEnglish (US)
Pages (from-to)6778-6784
Number of pages7
JournalJournal of the American Chemical Society
Volume108
Issue number21
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Globosides
Glycolipids
Oligosaccharides
Structural analysis
Conformations
Nuclear magnetic resonance
Molecules
Experiments

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Structural Analysis of a Glycolipid Head Group with One- and Two-State NMR Pseudoenergy Approaches. / Scarsdale, J. N.; Prestegard, J. H.; Yu, Robert K.

In: Journal of the American Chemical Society, Vol. 108, No. 21, 01.01.1986, p. 6778-6784.

Research output: Contribution to journalArticle

@article{a2009ea5339344f0893acd0c7b57d265,
title = "Structural Analysis of a Glycolipid Head Group with One- and Two-State NMR Pseudoenergy Approaches",
abstract = "The solution conformation of the oligosaccharide head group of the glycolipid globoside has been determined with the use of 1 H two-dimensional NMR methods. The intensities of cross peaks in cross relaxation correlated experiments have been analyzed in terms of interproton distances, and the distances have been incorporated as a pseudoenergy in a conformational energy calculation. Minimum energy conformations have been determined by using both one- and two-state models. Conformations are consistent with a previously proposed “L” shape for the molecule. Comparison of the results from the two models suggests the possibility of conformational flexibility to exist for the terminal residue of the head group.",
author = "Scarsdale, {J. N.} and Prestegard, {J. H.} and Yu, {Robert K}",
year = "1986",
month = "1",
day = "1",
doi = "10.1021/ja00281a053",
language = "English (US)",
volume = "108",
pages = "6778--6784",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Structural Analysis of a Glycolipid Head Group with One- and Two-State NMR Pseudoenergy Approaches

AU - Scarsdale, J. N.

AU - Prestegard, J. H.

AU - Yu, Robert K

PY - 1986/1/1

Y1 - 1986/1/1

N2 - The solution conformation of the oligosaccharide head group of the glycolipid globoside has been determined with the use of 1 H two-dimensional NMR methods. The intensities of cross peaks in cross relaxation correlated experiments have been analyzed in terms of interproton distances, and the distances have been incorporated as a pseudoenergy in a conformational energy calculation. Minimum energy conformations have been determined by using both one- and two-state models. Conformations are consistent with a previously proposed “L” shape for the molecule. Comparison of the results from the two models suggests the possibility of conformational flexibility to exist for the terminal residue of the head group.

AB - The solution conformation of the oligosaccharide head group of the glycolipid globoside has been determined with the use of 1 H two-dimensional NMR methods. The intensities of cross peaks in cross relaxation correlated experiments have been analyzed in terms of interproton distances, and the distances have been incorporated as a pseudoenergy in a conformational energy calculation. Minimum energy conformations have been determined by using both one- and two-state models. Conformations are consistent with a previously proposed “L” shape for the molecule. Comparison of the results from the two models suggests the possibility of conformational flexibility to exist for the terminal residue of the head group.

UR - http://www.scopus.com/inward/record.url?scp=0343336467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343336467&partnerID=8YFLogxK

U2 - 10.1021/ja00281a053

DO - 10.1021/ja00281a053

M3 - Article

AN - SCOPUS:0343336467

VL - 108

SP - 6778

EP - 6784

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 21

ER -