Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin

Ryan B. Turner, Lin Liu, Irina Yurievna Sazonova, Guy L. Reed

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

There is remarkable homology between the core structures of plasmin, a fibrin clot-degrading enzyme, and factor D, a complement-activating enzyme, despite markedly different biological functions. We postulated that sequence divergence in the loop structures between these two enzymes mediated the unique substrate and inhibitor interactions of plasmin. Recombinant microplasminogens chimerized with factor D sequences at loops 3, 5, and 7 were cleaved by the plasminogen activator urokinase and developed titratable active sites. Chimerization abolished functional interactions with the plasminogen activator streptokinase but did not block complex formation. The microplasmin chimeras showed enhanced resistance (ki decreased up to two to three times) to inactivation of microplasmin by α2-antiplasmin. Microplasmin chimerization had minimal (∼2 fold) effects on the catalytic efficiency for cleavage of small substrates and did not alter the cleavage of fibrin. However, microplasmin and the microplasmin chimeras showed enhanced abilities to degrade fibrin in plasma clots suspended in human plasma. These studies indicate that loop regions of the protease domain of plasmin are important for interactions with substrates, regulatory molecules, and inhibitors. Because modification of these regions affected substrate and inhibitor interactions, loop chimerization may hold promise for improving the clot dissolving properties of this enzyme.

Original languageEnglish (US)
Pages (from-to)33068-33074
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number36
DOIs
StatePublished - Sep 6 2002

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Fibrinolysin
Substrate Specificity
Fibrin
Complement Factor D
Substrates
Enzymes
Antifibrinolytic Agents
Plasminogen Activators
Complement Activating Enzymes
Plasma (human)
Streptokinase
Urokinase-Type Plasminogen Activator
Catalytic Domain
Peptide Hydrolases
microplasmin
Plasmas
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin. / Turner, Ryan B.; Liu, Lin; Sazonova, Irina Yurievna; Reed, Guy L.

In: Journal of Biological Chemistry, Vol. 277, No. 36, 06.09.2002, p. 33068-33074.

Research output: Contribution to journalArticle

Turner, Ryan B. ; Liu, Lin ; Sazonova, Irina Yurievna ; Reed, Guy L. / Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 36. pp. 33068-33074.
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