Structural rearrangements of the WT1 gene in Wilm's tumour cells

John Kenneth Cowell, R. B. Wadey, D. A. Haber, K. M. Call, D. E. Housman, J. Pritchard

Research output: Contribution to journalArticle

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Abstract

We have analysed 55 Wilms' tumour DNAs using the cDNA from the candidate Wilms' predisposition gene, WT1. One tumour, GOS 129, shows a partial homozygous deletion involving only the 3'-most exon of the gene. An adjacent 3' DNA sequence, J7-18, which lies on the same NotI fragment as WT1, is present in GOS 129. Thus, this partial deletion does not extend to the adjacent unmethylated 3' HTF island. These data support the candidature of WT1 as a Wilms' predisposition gene. Tumour GOS 129 has become homozygous as a result of a mitotic recombination event proximal to WT1. Three other tumours showed abnormally sized bands on Southern blot analysis which appear to reflect internal heterozygous rearrangements involving the 5' end of the gene. One of these tumours was from a bilaterally affected patient and the other 3 were from stage III or IV tumours.

Original languageEnglish (US)
Pages (from-to)595-599
Number of pages5
JournalOncogene
Volume6
Issue number4
StatePublished - May 28 1991
Externally publishedYes

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Gene Rearrangement
Wilms' Tumor Genes
Neoplasms
Wilms Tumor
Southern Blotting
Islands
Genetic Recombination
Genes
Exons
Complementary DNA
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Cowell, J. K., Wadey, R. B., Haber, D. A., Call, K. M., Housman, D. E., & Pritchard, J. (1991). Structural rearrangements of the WT1 gene in Wilm's tumour cells. Oncogene, 6(4), 595-599.

Structural rearrangements of the WT1 gene in Wilm's tumour cells. / Cowell, John Kenneth; Wadey, R. B.; Haber, D. A.; Call, K. M.; Housman, D. E.; Pritchard, J.

In: Oncogene, Vol. 6, No. 4, 28.05.1991, p. 595-599.

Research output: Contribution to journalArticle

Cowell, JK, Wadey, RB, Haber, DA, Call, KM, Housman, DE & Pritchard, J 1991, 'Structural rearrangements of the WT1 gene in Wilm's tumour cells', Oncogene, vol. 6, no. 4, pp. 595-599.
Cowell JK, Wadey RB, Haber DA, Call KM, Housman DE, Pritchard J. Structural rearrangements of the WT1 gene in Wilm's tumour cells. Oncogene. 1991 May 28;6(4):595-599.
Cowell, John Kenneth ; Wadey, R. B. ; Haber, D. A. ; Call, K. M. ; Housman, D. E. ; Pritchard, J. / Structural rearrangements of the WT1 gene in Wilm's tumour cells. In: Oncogene. 1991 ; Vol. 6, No. 4. pp. 595-599.
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