Structural requirements for the binding of modified proteins to the scavenger receptor of macrophages

Hanfang Zhang, Yuzhou Yang, Urs P. Steinbrecher

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Oxidized low density lipoprotein (LDL) and certain chemically modified LDL are recognized by the scavenger receptor of macrophages. All of these modifications involve charge-neutralizing derivatization of lysine amino groups. However, it remains controversial whether recognition of modified LDL by this receptor is due to the modification per se, or to other factors such as a conformational change of apoB. In this study, LDL and other proteins including bovine serum albumin, human high density lipoprotein, and murine IgG were derivatized with oxidation products generated from arachidonic acid by thermal autoxidation. Modified proteins had increased negative charge, and showed a more than 10-fold enhancement of degradation by mouse peritoneal macrophages via the scavenger receptor pathway. Modification was prevented by blocking lysine residues of the proteins by prior reductive methylation. Amino acid analysis revealed dose-dependent modification of lysine residues with no significant effects on any other amino acid. Fab fragments of monoclonal antibodies specific for adducts of oxidation products with lysine prevented the uptake of oxidation product-modified LDL and oxidized LDL by macrophages. Chromatography of oxidation product-modified LDL over Sepharose CL-4B showed that uptake by macrophages did not require LDL aggregation. These results suggest that a relatively simple domain consisting of a cluster of suitably derivatized lysine residues is sufficient for recognition by the scavenger receptor of macrophages.

Original languageEnglish (US)
Pages (from-to)5535-5542
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number8
StatePublished - Mar 15 1993

Fingerprint

Scavenger Receptors
LDL Lipoproteins
Lysine
Carrier Proteins
Macrophages
Oxidation
Proteins
Amino Acids
Lipoproteins
Immunoglobulin Fab Fragments
Methylation
LDL Receptors
Apolipoproteins B
Peritoneal Macrophages
HDL Lipoproteins
Bovine Serum Albumin
Chromatography
Arachidonic Acid
Agglomeration
Immunoglobulin G

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structural requirements for the binding of modified proteins to the scavenger receptor of macrophages. / Zhang, Hanfang; Yang, Yuzhou; Steinbrecher, Urs P.

In: Journal of Biological Chemistry, Vol. 268, No. 8, 15.03.1993, p. 5535-5542.

Research output: Contribution to journalArticle

Zhang, Hanfang ; Yang, Yuzhou ; Steinbrecher, Urs P. / Structural requirements for the binding of modified proteins to the scavenger receptor of macrophages. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 8. pp. 5535-5542.
@article{c0c4b308e1c645f39c9fe5bd04cd5cd2,
title = "Structural requirements for the binding of modified proteins to the scavenger receptor of macrophages",
abstract = "Oxidized low density lipoprotein (LDL) and certain chemically modified LDL are recognized by the scavenger receptor of macrophages. All of these modifications involve charge-neutralizing derivatization of lysine amino groups. However, it remains controversial whether recognition of modified LDL by this receptor is due to the modification per se, or to other factors such as a conformational change of apoB. In this study, LDL and other proteins including bovine serum albumin, human high density lipoprotein, and murine IgG were derivatized with oxidation products generated from arachidonic acid by thermal autoxidation. Modified proteins had increased negative charge, and showed a more than 10-fold enhancement of degradation by mouse peritoneal macrophages via the scavenger receptor pathway. Modification was prevented by blocking lysine residues of the proteins by prior reductive methylation. Amino acid analysis revealed dose-dependent modification of lysine residues with no significant effects on any other amino acid. Fab fragments of monoclonal antibodies specific for adducts of oxidation products with lysine prevented the uptake of oxidation product-modified LDL and oxidized LDL by macrophages. Chromatography of oxidation product-modified LDL over Sepharose CL-4B showed that uptake by macrophages did not require LDL aggregation. These results suggest that a relatively simple domain consisting of a cluster of suitably derivatized lysine residues is sufficient for recognition by the scavenger receptor of macrophages.",
author = "Hanfang Zhang and Yuzhou Yang and Steinbrecher, {Urs P.}",
year = "1993",
month = "3",
day = "15",
language = "English (US)",
volume = "268",
pages = "5535--5542",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "8",

}

TY - JOUR

T1 - Structural requirements for the binding of modified proteins to the scavenger receptor of macrophages

AU - Zhang, Hanfang

AU - Yang, Yuzhou

AU - Steinbrecher, Urs P.

PY - 1993/3/15

Y1 - 1993/3/15

N2 - Oxidized low density lipoprotein (LDL) and certain chemically modified LDL are recognized by the scavenger receptor of macrophages. All of these modifications involve charge-neutralizing derivatization of lysine amino groups. However, it remains controversial whether recognition of modified LDL by this receptor is due to the modification per se, or to other factors such as a conformational change of apoB. In this study, LDL and other proteins including bovine serum albumin, human high density lipoprotein, and murine IgG were derivatized with oxidation products generated from arachidonic acid by thermal autoxidation. Modified proteins had increased negative charge, and showed a more than 10-fold enhancement of degradation by mouse peritoneal macrophages via the scavenger receptor pathway. Modification was prevented by blocking lysine residues of the proteins by prior reductive methylation. Amino acid analysis revealed dose-dependent modification of lysine residues with no significant effects on any other amino acid. Fab fragments of monoclonal antibodies specific for adducts of oxidation products with lysine prevented the uptake of oxidation product-modified LDL and oxidized LDL by macrophages. Chromatography of oxidation product-modified LDL over Sepharose CL-4B showed that uptake by macrophages did not require LDL aggregation. These results suggest that a relatively simple domain consisting of a cluster of suitably derivatized lysine residues is sufficient for recognition by the scavenger receptor of macrophages.

AB - Oxidized low density lipoprotein (LDL) and certain chemically modified LDL are recognized by the scavenger receptor of macrophages. All of these modifications involve charge-neutralizing derivatization of lysine amino groups. However, it remains controversial whether recognition of modified LDL by this receptor is due to the modification per se, or to other factors such as a conformational change of apoB. In this study, LDL and other proteins including bovine serum albumin, human high density lipoprotein, and murine IgG were derivatized with oxidation products generated from arachidonic acid by thermal autoxidation. Modified proteins had increased negative charge, and showed a more than 10-fold enhancement of degradation by mouse peritoneal macrophages via the scavenger receptor pathway. Modification was prevented by blocking lysine residues of the proteins by prior reductive methylation. Amino acid analysis revealed dose-dependent modification of lysine residues with no significant effects on any other amino acid. Fab fragments of monoclonal antibodies specific for adducts of oxidation products with lysine prevented the uptake of oxidation product-modified LDL and oxidized LDL by macrophages. Chromatography of oxidation product-modified LDL over Sepharose CL-4B showed that uptake by macrophages did not require LDL aggregation. These results suggest that a relatively simple domain consisting of a cluster of suitably derivatized lysine residues is sufficient for recognition by the scavenger receptor of macrophages.

UR - http://www.scopus.com/inward/record.url?scp=0027520775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027520775&partnerID=8YFLogxK

M3 - Article

VL - 268

SP - 5535

EP - 5542

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 8

ER -