Structure and expression of the FlaA periplasmic flagellar protein of Borrelia burgdorferi

Yigong Ge, Chunhao Li, Linda Corum, Clive A. Slaughter, Nyles W. Charon

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The spirochete which causes Lyme disease, Borrelia burgdorferi, has many features common to other spirochete species. Outermost is a membrane sheath, and within this sheath are the cell cylinder and periplasmic flagella (PFs). The PFs are subterminally attached to the cell cylinder and overlap in the center of the cell. Most descriptions of the B. burgdorferi flagellar filaments indicate that these organelles consist of only one flagellin protein (FlaB). In contrast, the PFs from other spirochete species are comprised of an outer layer of FlaA and a core of FlaB. We recently found that a flaA homolog was expressed in B. burgdorferi and that it mapped in a fla/che operon. These results led us to analyze the PFs and FlaA or B. burgdorferi in detail. Using Triton X-100 to remove the outer membrane and isolate the PFs, we found that the 38.0-kDa FlaA protein purified with the PFs in association with the 41.0-kDa FlaB protein. On the other hand, purifying the PFs by using Sarkosyl resulted in no FlaA in the isolated PFs. Sarkosyl has been used by others to purify B. burgdorferi PFs, and our results explain in part their failure to find FlaA. Unlike other spirochetes, B. burgdorferi FlaA was expressed at a lower level than FlaB. In characterizing FlaA, we found that it was posttranslationally modified by glycosylation, and thus it resembles its counterpart from Serpulina hyodysenteriae. We also tested if FlaA was synthesized in a spontaneously occurring PF mutant orB. burgdorferi (HB19Fla-). Although this mutant still synthesized FlaA message in amounts similar to the wild-type amounts, it failed to synthesize FlaA protein. These results suggest that, in agreement with data found for FlaB and other spirochete flagellar proteins, FlaA is likely to be regulated on the translational level. Western blot analysis using Treponema pallidum anti-FlaA serum indicated that FlaA was antigenically well conserved in several spirochete species. Taken together, the results indicate that both FlaA and FlaB comprise the PFs of B. burgdorferi and that they are regulated differently from flagellin proteins of other bacteria.

Original languageEnglish (US)
Pages (from-to)2418-2425
Number of pages8
JournalJournal of Bacteriology
Volume180
Issue number9
StatePublished - May 1 1998

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Periplasmic Proteins
Borrelia burgdorferi
Flagella
Spirochaetales
Proteins
Brachyspira hyodysenteriae
Flagellin
Treponema pallidum
Membranes
Lyme Disease
Octoxynol
Operon
Glycosylation
Organelles

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Structure and expression of the FlaA periplasmic flagellar protein of Borrelia burgdorferi. / Ge, Yigong; Li, Chunhao; Corum, Linda; Slaughter, Clive A.; Charon, Nyles W.

In: Journal of Bacteriology, Vol. 180, No. 9, 01.05.1998, p. 2418-2425.

Research output: Contribution to journalArticle

Ge, Yigong ; Li, Chunhao ; Corum, Linda ; Slaughter, Clive A. ; Charon, Nyles W. / Structure and expression of the FlaA periplasmic flagellar protein of Borrelia burgdorferi. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 9. pp. 2418-2425.
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abstract = "The spirochete which causes Lyme disease, Borrelia burgdorferi, has many features common to other spirochete species. Outermost is a membrane sheath, and within this sheath are the cell cylinder and periplasmic flagella (PFs). The PFs are subterminally attached to the cell cylinder and overlap in the center of the cell. Most descriptions of the B. burgdorferi flagellar filaments indicate that these organelles consist of only one flagellin protein (FlaB). In contrast, the PFs from other spirochete species are comprised of an outer layer of FlaA and a core of FlaB. We recently found that a flaA homolog was expressed in B. burgdorferi and that it mapped in a fla/che operon. These results led us to analyze the PFs and FlaA or B. burgdorferi in detail. Using Triton X-100 to remove the outer membrane and isolate the PFs, we found that the 38.0-kDa FlaA protein purified with the PFs in association with the 41.0-kDa FlaB protein. On the other hand, purifying the PFs by using Sarkosyl resulted in no FlaA in the isolated PFs. Sarkosyl has been used by others to purify B. burgdorferi PFs, and our results explain in part their failure to find FlaA. Unlike other spirochetes, B. burgdorferi FlaA was expressed at a lower level than FlaB. In characterizing FlaA, we found that it was posttranslationally modified by glycosylation, and thus it resembles its counterpart from Serpulina hyodysenteriae. We also tested if FlaA was synthesized in a spontaneously occurring PF mutant orB. burgdorferi (HB19Fla-). Although this mutant still synthesized FlaA message in amounts similar to the wild-type amounts, it failed to synthesize FlaA protein. These results suggest that, in agreement with data found for FlaB and other spirochete flagellar proteins, FlaA is likely to be regulated on the translational level. Western blot analysis using Treponema pallidum anti-FlaA serum indicated that FlaA was antigenically well conserved in several spirochete species. Taken together, the results indicate that both FlaA and FlaB comprise the PFs of B. burgdorferi and that they are regulated differently from flagellin proteins of other bacteria.",
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