Structure and expression of the murine calcyon gene

Rujuan Dai, Clare M Bergson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Calcyon was recently identified as a D1 dopamine receptor (DR1) interacting protein. Previous studies show that calcyon can potentiate DR1 mediated intracellular Ca 2+ release in transfected HEK293 cells, and may play an important role in DR1 Ca 2+ signaling in brain. We report that similar to the genomic structure of the human gene, the mouse calcyon gene contains six relatively short exons, with a large intron (about 8.4 kb) between exons one and two. The mouse and human calcyon genes exhibit a high level of sequence homology (77.5% at the nucleotide level) within coding regions. Northern blot and RT-PCR analyses reveal that mouse calcyon transcripts are most abundant in brain, but also present in testis and ovary, as well as in kidney and heart at much lower levels. The most distal of the transcript initiation sites identified by 5′ RACE is located 159 nucleotides upstream of the putative start of translation. BLAST search of the NCBI mouse EST database and RT-PCR analysis uncovered two differentially spliced transcripts, 'mcal-A' and 'mcal-B.' The two transcripts are identical, except that mcal-B contains a longer 3′ untranslated region due to retention of a short intron (I5) between exons five and six. However, mcal-A represents the predominant calcyon transcript in mouse tissue. Further, the presence of I5 produced no detectable differences in the biosynthesis of calcyon polypeptide when expressed in HEK293, MDCK and Neuro2a cells.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalGene
Volume311
Issue number1-2
DOIs
StatePublished - Jul 12 2003

Fingerprint

Genes
Exons
Introns
Nucleotides
Receptor-Interacting Protein Serine-Threonine Kinases
Polymerase Chain Reaction
Madin Darby Canine Kidney Cells
HEK293 Cells
Expressed Sequence Tags
Brain
3' Untranslated Regions
Sequence Homology
calcyon
Northern Blotting
Testis
Ovary
Dopamine
Databases
Kidney
Peptides

Keywords

  • Dopamine receptor interacting protein
  • Exon
  • Intron
  • Mouse
  • Splicing
  • Transcription start site

ASJC Scopus subject areas

  • Genetics

Cite this

Structure and expression of the murine calcyon gene. / Dai, Rujuan; Bergson, Clare M.

In: Gene, Vol. 311, No. 1-2, 12.07.2003, p. 111-117.

Research output: Contribution to journalArticle

Dai, Rujuan ; Bergson, Clare M. / Structure and expression of the murine calcyon gene. In: Gene. 2003 ; Vol. 311, No. 1-2. pp. 111-117.
@article{4ce20c56ed1d4df68b9388e5932a1ac7,
title = "Structure and expression of the murine calcyon gene",
abstract = "Calcyon was recently identified as a D1 dopamine receptor (DR1) interacting protein. Previous studies show that calcyon can potentiate DR1 mediated intracellular Ca 2+ release in transfected HEK293 cells, and may play an important role in DR1 Ca 2+ signaling in brain. We report that similar to the genomic structure of the human gene, the mouse calcyon gene contains six relatively short exons, with a large intron (about 8.4 kb) between exons one and two. The mouse and human calcyon genes exhibit a high level of sequence homology (77.5{\%} at the nucleotide level) within coding regions. Northern blot and RT-PCR analyses reveal that mouse calcyon transcripts are most abundant in brain, but also present in testis and ovary, as well as in kidney and heart at much lower levels. The most distal of the transcript initiation sites identified by 5′ RACE is located 159 nucleotides upstream of the putative start of translation. BLAST search of the NCBI mouse EST database and RT-PCR analysis uncovered two differentially spliced transcripts, 'mcal-A' and 'mcal-B.' The two transcripts are identical, except that mcal-B contains a longer 3′ untranslated region due to retention of a short intron (I5) between exons five and six. However, mcal-A represents the predominant calcyon transcript in mouse tissue. Further, the presence of I5 produced no detectable differences in the biosynthesis of calcyon polypeptide when expressed in HEK293, MDCK and Neuro2a cells.",
keywords = "Dopamine receptor interacting protein, Exon, Intron, Mouse, Splicing, Transcription start site",
author = "Rujuan Dai and Bergson, {Clare M}",
year = "2003",
month = "7",
day = "12",
doi = "10.1016/S0378-1119(03)00564-X",
language = "English (US)",
volume = "311",
pages = "111--117",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Structure and expression of the murine calcyon gene

AU - Dai, Rujuan

AU - Bergson, Clare M

PY - 2003/7/12

Y1 - 2003/7/12

N2 - Calcyon was recently identified as a D1 dopamine receptor (DR1) interacting protein. Previous studies show that calcyon can potentiate DR1 mediated intracellular Ca 2+ release in transfected HEK293 cells, and may play an important role in DR1 Ca 2+ signaling in brain. We report that similar to the genomic structure of the human gene, the mouse calcyon gene contains six relatively short exons, with a large intron (about 8.4 kb) between exons one and two. The mouse and human calcyon genes exhibit a high level of sequence homology (77.5% at the nucleotide level) within coding regions. Northern blot and RT-PCR analyses reveal that mouse calcyon transcripts are most abundant in brain, but also present in testis and ovary, as well as in kidney and heart at much lower levels. The most distal of the transcript initiation sites identified by 5′ RACE is located 159 nucleotides upstream of the putative start of translation. BLAST search of the NCBI mouse EST database and RT-PCR analysis uncovered two differentially spliced transcripts, 'mcal-A' and 'mcal-B.' The two transcripts are identical, except that mcal-B contains a longer 3′ untranslated region due to retention of a short intron (I5) between exons five and six. However, mcal-A represents the predominant calcyon transcript in mouse tissue. Further, the presence of I5 produced no detectable differences in the biosynthesis of calcyon polypeptide when expressed in HEK293, MDCK and Neuro2a cells.

AB - Calcyon was recently identified as a D1 dopamine receptor (DR1) interacting protein. Previous studies show that calcyon can potentiate DR1 mediated intracellular Ca 2+ release in transfected HEK293 cells, and may play an important role in DR1 Ca 2+ signaling in brain. We report that similar to the genomic structure of the human gene, the mouse calcyon gene contains six relatively short exons, with a large intron (about 8.4 kb) between exons one and two. The mouse and human calcyon genes exhibit a high level of sequence homology (77.5% at the nucleotide level) within coding regions. Northern blot and RT-PCR analyses reveal that mouse calcyon transcripts are most abundant in brain, but also present in testis and ovary, as well as in kidney and heart at much lower levels. The most distal of the transcript initiation sites identified by 5′ RACE is located 159 nucleotides upstream of the putative start of translation. BLAST search of the NCBI mouse EST database and RT-PCR analysis uncovered two differentially spliced transcripts, 'mcal-A' and 'mcal-B.' The two transcripts are identical, except that mcal-B contains a longer 3′ untranslated region due to retention of a short intron (I5) between exons five and six. However, mcal-A represents the predominant calcyon transcript in mouse tissue. Further, the presence of I5 produced no detectable differences in the biosynthesis of calcyon polypeptide when expressed in HEK293, MDCK and Neuro2a cells.

KW - Dopamine receptor interacting protein

KW - Exon

KW - Intron

KW - Mouse

KW - Splicing

KW - Transcription start site

UR - http://www.scopus.com/inward/record.url?scp=0037633401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037633401&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(03)00564-X

DO - 10.1016/S0378-1119(03)00564-X

M3 - Article

VL - 311

SP - 111

EP - 117

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -