Studies on antitumor components of Collybia confluens

S. H. Kim, J. S. Kim, M. R. Jin, H. W. Kim, E. C. Choi, B. K. Kim

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To find antitumor components from higher fungi, the mycelia of Collybia confluens (Pers. ex Fr.) Kummer were cultured in artificial media. For efficient production of the mycelia, the influences of various modifications of culture conditions were examined. A water-soluble protein-bound polysaccharide fraction, Fr. A, was obtained frcm the mycelia by hot water extraction. When Fr. A was purified and fractionated by DEAE-cellulose and Sephadex G-200 gel filtration chromatographies into four fractions which were designated B, C, C-I and C-II. The tumor inhibition ratios of these fractions ranged from 46% to 75% against the solid forms of sarcoma 180 in ICR mice at doses of 20 and 50 mg/kg/day when given intraperitoneally. Especially, Fr. C which was named Collyban (CB) exhibited a marked life-prolonging effect of the mice against ascitic forms of sarcoma 180 at a dose of 50 mg via i.p. administration. To extend spectra of the antitumor activities and eliminate the effects of allograft rejection, the characterisation of antitumor effects of CB was performed in syngeneic host-tumor systems. It did not show any antitumor activity against L1210 murine leukemia in CD2F1 mice but prolonged their life span against ascitic forms of MM46 carcinoma in C3H/He mice. Also it exhibited antitumor activity against human cervical cancer HeLa cells that were xenografted into nude mice having BALB/c genetic backgrounds by the i.p. injection at a dose of 100 mg/kg/day. In order to characterize the antitumor components, CB was examined by chemical analysis. It was acidic protein-bound polysaccharides composed of 31% polysaccharide, 27% protein and 3% hexosamine. CB was fractionated into two fractions, Fr. C-I (M.W.: 500 Kd) and Fr. C-II (M.W.: 30 and 8 Kd) by Sephadex G-200 gel filtration chromatography.

Original languageEnglish (US)
Pages (from-to)267-281
Number of pages15
JournalKorean Journal of Pharmacognosy
Volume24
Issue number4
StatePublished - Dec 1 1993
Externally publishedYes

Keywords

  • antitumor component
  • basidomycetes
  • Collyban
  • Collybia confluens
  • cultured mycelia
  • HeLa cell
  • immunomodulator
  • protein-bound polysaccharides

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

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    Kim, S. H., Kim, J. S., Jin, M. R., Kim, H. W., Choi, E. C., & Kim, B. K. (1993). Studies on antitumor components of Collybia confluens. Korean Journal of Pharmacognosy, 24(4), 267-281.