Studies on Inhibition of Activated Oncogene Expression by Antisense RNA

Qiwei Yang, Binggang Sun, Yawen Chen, Yuanshu Dong, Defeng Chen

Research output: Contribution to journalArticle

Abstract

In this paper, the sense and antisense mammalian expression vectors of T24-ras activated oncogene were introduced into cultured NIH3T3 cells in sense→antisense and antisense → sense orders. The transfected cells were selected by drug selection. The morphology change, Southern hybridization, frequency of soft agar colonies and the detection of P21 amount were investigated. The results showed that the sense recombinant could cause the malignant transformation of NIH3T3 cells while the antisense recombinant could inhibite this kind of transformation. Furthermore, the inhibitory effect varied according to the different transfected orders.

Original languageEnglish (US)
Pages (from-to)247-248
Number of pages2
JournalActa Genetica Sinica
Volume23
Issue number3
StatePublished - Dec 1 1996

Fingerprint

Antisense RNA
Oncogenes
ras Genes
Agar
Cultured Cells
Pharmaceutical Preparations

Keywords

  • Antisense RNA
  • DNA transfection
  • Oncogene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, Q., Sun, B., Chen, Y., Dong, Y., & Chen, D. (1996). Studies on Inhibition of Activated Oncogene Expression by Antisense RNA. Acta Genetica Sinica, 23(3), 247-248.

Studies on Inhibition of Activated Oncogene Expression by Antisense RNA. / Yang, Qiwei; Sun, Binggang; Chen, Yawen; Dong, Yuanshu; Chen, Defeng.

In: Acta Genetica Sinica, Vol. 23, No. 3, 01.12.1996, p. 247-248.

Research output: Contribution to journalArticle

Yang, Q, Sun, B, Chen, Y, Dong, Y & Chen, D 1996, 'Studies on Inhibition of Activated Oncogene Expression by Antisense RNA', Acta Genetica Sinica, vol. 23, no. 3, pp. 247-248.
Yang Q, Sun B, Chen Y, Dong Y, Chen D. Studies on Inhibition of Activated Oncogene Expression by Antisense RNA. Acta Genetica Sinica. 1996 Dec 1;23(3):247-248.
Yang, Qiwei ; Sun, Binggang ; Chen, Yawen ; Dong, Yuanshu ; Chen, Defeng. / Studies on Inhibition of Activated Oncogene Expression by Antisense RNA. In: Acta Genetica Sinica. 1996 ; Vol. 23, No. 3. pp. 247-248.
@article{db76b4a632b548ea93271c0fd876162d,
title = "Studies on Inhibition of Activated Oncogene Expression by Antisense RNA",
abstract = "In this paper, the sense and antisense mammalian expression vectors of T24-ras activated oncogene were introduced into cultured NIH3T3 cells in sense→antisense and antisense → sense orders. The transfected cells were selected by drug selection. The morphology change, Southern hybridization, frequency of soft agar colonies and the detection of P21 amount were investigated. The results showed that the sense recombinant could cause the malignant transformation of NIH3T3 cells while the antisense recombinant could inhibite this kind of transformation. Furthermore, the inhibitory effect varied according to the different transfected orders.",
keywords = "Antisense RNA, DNA transfection, Oncogene",
author = "Qiwei Yang and Binggang Sun and Yawen Chen and Yuanshu Dong and Defeng Chen",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "23",
pages = "247--248",
journal = "Journal of Genetics and Genomics",
issn = "1673-8527",
publisher = "Institute of Genetics and Developmental Biology",
number = "3",

}

TY - JOUR

T1 - Studies on Inhibition of Activated Oncogene Expression by Antisense RNA

AU - Yang, Qiwei

AU - Sun, Binggang

AU - Chen, Yawen

AU - Dong, Yuanshu

AU - Chen, Defeng

PY - 1996/12/1

Y1 - 1996/12/1

N2 - In this paper, the sense and antisense mammalian expression vectors of T24-ras activated oncogene were introduced into cultured NIH3T3 cells in sense→antisense and antisense → sense orders. The transfected cells were selected by drug selection. The morphology change, Southern hybridization, frequency of soft agar colonies and the detection of P21 amount were investigated. The results showed that the sense recombinant could cause the malignant transformation of NIH3T3 cells while the antisense recombinant could inhibite this kind of transformation. Furthermore, the inhibitory effect varied according to the different transfected orders.

AB - In this paper, the sense and antisense mammalian expression vectors of T24-ras activated oncogene were introduced into cultured NIH3T3 cells in sense→antisense and antisense → sense orders. The transfected cells were selected by drug selection. The morphology change, Southern hybridization, frequency of soft agar colonies and the detection of P21 amount were investigated. The results showed that the sense recombinant could cause the malignant transformation of NIH3T3 cells while the antisense recombinant could inhibite this kind of transformation. Furthermore, the inhibitory effect varied according to the different transfected orders.

KW - Antisense RNA

KW - DNA transfection

KW - Oncogene

UR - http://www.scopus.com/inward/record.url?scp=0030333417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030333417&partnerID=8YFLogxK

M3 - Article

C2 - 8950853

AN - SCOPUS:0030333417

VL - 23

SP - 247

EP - 248

JO - Journal of Genetics and Genomics

JF - Journal of Genetics and Genomics

SN - 1673-8527

IS - 3

ER -