Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact

H. Zhang, W. B. Davis, X. Chen, R. L. Whisler, D. G. Cornwell

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Low density lipoproteins (LDL), isolated by ultracentrifugal flotation, were oxidized (LDL(OXID)) slowly during dialysis against 0.15 M NaCl and subsequent incubation in 96% air-4% CO2 at 37°C. Butylated hydroxytoluene prevented LDL oxidation. LDL preparations from different sera were oxidized at different rates and the degree of lipid peroxidation was controlled by varying the incubation time. Mild oxidation did not alter the electrophoretic mobility of the LDL(OXID) preparation. LDL(OXID) contained lipid peroxides in neutral lipids, had increased amounts of lysophosphatidylcholine, and contained a number of complex oxidation products that were generated from the oxidation of free fatty acids. These oxidation products included large amounts of soluble material that cross-reacted with antibodies to PGE2 but not 6-keto-PGF(1α). The amount of cross-reacting material was proportional to the degree of lipid peroxidation. Cross-reacting material in LDL(OXID) preparations was evidently formed from the oxidation of free fatty acids released from LDL, since cross-reacting material was also formed when a synthetic fat emulsion was oxidized in the presence of free arachidonic acid.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalJournal of Lipid Research
Volume30
Issue number2
StatePublished - Jan 1 1989

Fingerprint

LDL Lipoproteins
Artifacts
Prostaglandins
Lipoproteins
Oxidation
Lipids
Nonesterified Fatty Acids
Lipid Peroxidation
Butylated Hydroxytoluene
Electrophoretic mobility
Lysophosphatidylcholines
Dialysis
Lipid Peroxides
Prostaglandins F
Flotation
Emulsions
Dinoprostone
Arachidonic Acid
oxidized low density lipoprotein
Fats

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. / Zhang, H.; Davis, W. B.; Chen, X.; Whisler, R. L.; Cornwell, D. G.

In: Journal of Lipid Research, Vol. 30, No. 2, 01.01.1989, p. 141-148.

Research output: Contribution to journalArticle

Zhang, H. ; Davis, W. B. ; Chen, X. ; Whisler, R. L. ; Cornwell, D. G. / Studies on oxidized low density lipoproteins. Controlled oxidation and a prostaglandin artifact. In: Journal of Lipid Research. 1989 ; Vol. 30, No. 2. pp. 141-148.
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