Studies on protoplast formation and regeneration of Ganoderma lucidum

Seung Hee Choi, Byong Kak Kim, Ha Won Kim, Jin Hwan Kwak, Eung Chil Choi, Young Choong Kim, Young Bok Yoo, Yong Hwan Park

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

To obtain a new strain of Ganoderma lucidum by protoplast fusion technique, its protoplast formation and regeneration were studied. Several factors affecting the protoplast formation and regeneration were investigated to find their optimum conditions. The mycelium was grown for four days on the cellophane membrane placed on G. lucidum complete medium (GCM). When various commercial lytic enzymes were examined for protoplast isolation, the combination of Novozym 234 and β-glucuronidase was found to be effective. An osmotic stabilizer, 0.6 M sucrose in 20 mM phosphate buffer pH 5.8, gave the highest yield of protoplasts. Three-hour incubation in shaking incubator was most suitable for releasing protoplasts. To increase the protoplast yield, pretreatment with 2-mercaptoethanol was carried out. The regeneration frequency in GCM containing 0.6 M MgSO4·7H2O was shown to be 0.66%.

Original languageEnglish (US)
Pages (from-to)158-164
Number of pages7
JournalArchives of Pharmacal Research
Volume10
Issue number3
DOIs
StatePublished - Sep 1 1987

Keywords

  • Ganoderma lucidum
  • Lytic enzyme
  • Osmotic stabilizer
  • Polyporaceae
  • Protoplast
  • Regeneration frequency

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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    Choi, S. H., Kim, B. K., Kim, H. W., Kwak, J. H., Choi, E. C., Kim, Y. C., Yoo, Y. B., & Park, Y. H. (1987). Studies on protoplast formation and regeneration of Ganoderma lucidum. Archives of Pharmacal Research, 10(3), 158-164. https://doi.org/10.1007/BF02861906