Numerous recent studies have suggested that the predicted cytosolic domains of G-protein-coupled receptors (GPCRs) represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G-proteins. This chapter describes two in vitro methods for ascertaining interactions between GPCRs and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.
|Original language||English (US)|
|Number of pages||8|
|Journal||Methods in molecular biology (Clifton, N.J.)|
|State||Published - 2004|
ASJC Scopus subject areas
- Molecular Biology