Abstract
Numerous recent studies have suggested that the predicted cytosolic domains of G-protein-coupled receptors (GPCRs) represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G-proteins. This chapter describes two in vitro methods for ascertaining interactions between GPCRs and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.
Original language | English (US) |
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Pages (from-to) | 371-378 |
Number of pages | 8 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 259 |
State | Published - 2004 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics