Study of G-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs.

Ashley E. Brady, Qin Wang, Lee E. Limbird

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Numerous recent studies have suggested that the predicted cytosolic domains of G-protein-coupled receptors (GPCRs) represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G-proteins. This chapter describes two in vitro methods for ascertaining interactions between GPCRs and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume259
StatePublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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