Abstract
Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.
Original language | English (US) |
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Pages (from-to) | 347-355 |
Number of pages | 9 |
Journal | Methods in Molecular Biology |
Volume | 746 |
DOIs | |
State | Published - 2011 |
Externally published | Yes |
Keywords
- G protein-coupled receptor
- Gel overlay
- Glutathione-S-transferase pull-down
- Protein-protein interaction
ASJC Scopus subject areas
- Molecular Biology
- Genetics