Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs

Ashley E. Brady, Yunjia Chen, Lee E. Limbird, Qin Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalMethods in Molecular Biology
Volume746
DOIs
StatePublished - 2011
Externally publishedYes

Keywords

  • G protein-coupled receptor
  • Gel overlay
  • Glutathione-S-transferase pull-down
  • Protein-protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs'. Together they form a unique fingerprint.

Cite this