Abstract
We report four new derivatization agents, acridone-N-acetic acid (ARC), carbazole-9-ylacetic acid (CRA), carbazole-9-ylpropionic acid (CRP), and 2- methyl-2-carbazole-9-ylacetic acid (MCRA), with strong fluorescence emission which has low dependence on solvent polarity. The emission maxima for ARC, CRA, CRP, and MCRA were 430 nm (λ(ex) 404 nm), 368 nm (λ(ex) 335 nm), 356 nm (λ(ex) 340 nm) and 360 nm (λ(ex) 330 nm), respectively. The effects of mobile-phase composition, pH, and temperature on the liquid chromatographic retention behavior of the four fluorescence agents were investigated. An experimental model was established for calculating the inclusion constants of cyclodextrin (CD) complexes in the dynamic state, using β-cyclodextrin (β- CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) as examples, and different mobile-phase compositions. On the basis of the model, the inclusion constants of the solutes in pure water (K(fw)) were determined by extrapolation. The thermodynamic parameters (ΔH°and ΔS°) and dissociation constants K(am) for the solutes in this chromatographic system were obtained by means of capacity factor (k) values using a corresponding model formulation.
Original language | English (US) |
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Pages (from-to) | 95-104 |
Number of pages | 10 |
Journal | Chromatographia |
Volume | 49 |
Issue number | 1-2 |
DOIs | |
State | Published - 1999 |
Externally published | Yes |
Keywords
- Column liquid chromatography
- Cyclodextrin additives
- Fluorescence agents
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry