Study of the chromatographic behavior and inclusion constants of new fluorescence agents by use of cyclodextrin additives under conditions of steady- and dynamic-state equilibrium and with fluorescence detection

J. M. You, W. J. Lao, X. J. Fan, Q. Y. Ou, X. L. Jia

Research output: Contribution to journalArticle

2 Scopus citations


We report four new derivatization agents, acridone-N-acetic acid (ARC), carbazole-9-ylacetic acid (CRA), carbazole-9-ylpropionic acid (CRP), and 2- methyl-2-carbazole-9-ylacetic acid (MCRA), with strong fluorescence emission which has low dependence on solvent polarity. The emission maxima for ARC, CRA, CRP, and MCRA were 430 nm (λ(ex) 404 nm), 368 nm (λ(ex) 335 nm), 356 nm (λ(ex) 340 nm) and 360 nm (λ(ex) 330 nm), respectively. The effects of mobile-phase composition, pH, and temperature on the liquid chromatographic retention behavior of the four fluorescence agents were investigated. An experimental model was established for calculating the inclusion constants of cyclodextrin (CD) complexes in the dynamic state, using β-cyclodextrin (β- CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) as examples, and different mobile-phase compositions. On the basis of the model, the inclusion constants of the solutes in pure water (K(fw)) were determined by extrapolation. The thermodynamic parameters (ΔH°and ΔS°) and dissociation constants K(am) for the solutes in this chromatographic system were obtained by means of capacity factor (k) values using a corresponding model formulation.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
Issue number1-2
Publication statusPublished - Jan 1 1999
Externally publishedYes



  • Column liquid chromatography
  • Cyclodextrin additives
  • Fluorescence agents

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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