Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

Zhi-Chun Ding, Qi Zheng, Bin Cai, Feng Yun Ni, Wen Hao Yu, Xing Chen Teng, Yuan Gao, Fang Liu, Dong Chen, Yang Wang, Hou Ming Wu, Hong Zhe Sun, Ming Jie Zhang, Xiang Shi Tan, Zhong Xian Huang

Research output: Contribution to journalShort survey

11 Scopus citations

Abstract

Human metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6 CPCP 9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation.

Original languageEnglish (US)
Pages (from-to)1965-1972
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume102
Issue number11
DOIs
StatePublished - Nov 1 2008

Keywords

  • Cell bioassay
  • Metallothionein (MT)
  • Mutation
  • Neuronal growth inhibitory factor (GIF)
  • Structure-function relationship

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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  • Cite this

    Ding, Z-C., Zheng, Q., Cai, B., Ni, F. Y., Yu, W. H., Teng, X. C., Gao, Y., Liu, F., Chen, D., Wang, Y., Wu, H. M., Sun, H. Z., Zhang, M. J., Tan, X. S., & Huang, Z. X. (2008). Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF). Journal of Inorganic Biochemistry, 102(11), 1965-1972. https://doi.org/10.1016/j.jinorgbio.2008.07.007