Subregion- and cell type-restricted gene knockout in mouse brain

Joseph Zhuo Tsien, Dong Feng Chen, David Gerber, Cindy Tom, Eric H. Mercer, David J. Anderson, Mark Mayford, Eric R. Kandel, Susumu Tonegawa

Research output: Contribution to journalArticle

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Abstract

Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Original languageEnglish (US)
Pages (from-to)1317-1326
Number of pages10
JournalCell
Volume87
Issue number7
DOIs
StatePublished - Dec 27 1996
Externally publishedYes

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Gene Knockout Techniques
Knockout Mice
Genetic Recombination
Brain
Genes
Bacteriophage P1
Hippocampal CA1 Region
Animal Behavior
Pyramidal Cells
Gene Deletion
Mutation
Bacteriophages
Animals
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tsien, J. Z., Chen, D. F., Gerber, D., Tom, C., Mercer, E. H., Anderson, D. J., ... Tonegawa, S. (1996). Subregion- and cell type-restricted gene knockout in mouse brain. Cell, 87(7), 1317-1326. https://doi.org/10.1016/S0092-8674(00)81826-7

Subregion- and cell type-restricted gene knockout in mouse brain. / Tsien, Joseph Zhuo; Chen, Dong Feng; Gerber, David; Tom, Cindy; Mercer, Eric H.; Anderson, David J.; Mayford, Mark; Kandel, Eric R.; Tonegawa, Susumu.

In: Cell, Vol. 87, No. 7, 27.12.1996, p. 1317-1326.

Research output: Contribution to journalArticle

Tsien, JZ, Chen, DF, Gerber, D, Tom, C, Mercer, EH, Anderson, DJ, Mayford, M, Kandel, ER & Tonegawa, S 1996, 'Subregion- and cell type-restricted gene knockout in mouse brain', Cell, vol. 87, no. 7, pp. 1317-1326. https://doi.org/10.1016/S0092-8674(00)81826-7
Tsien JZ, Chen DF, Gerber D, Tom C, Mercer EH, Anderson DJ et al. Subregion- and cell type-restricted gene knockout in mouse brain. Cell. 1996 Dec 27;87(7):1317-1326. https://doi.org/10.1016/S0092-8674(00)81826-7
Tsien, Joseph Zhuo ; Chen, Dong Feng ; Gerber, David ; Tom, Cindy ; Mercer, Eric H. ; Anderson, David J. ; Mayford, Mark ; Kandel, Eric R. ; Tonegawa, Susumu. / Subregion- and cell type-restricted gene knockout in mouse brain. In: Cell. 1996 ; Vol. 87, No. 7. pp. 1317-1326.
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