Subregion- and cell type-restricted gene knockout in mouse brain

Joe Z. Tsien, Dong Feng Chen, David Gerber, Cindy Tom, Eric H. Mercer, David J. Anderson, Mark Mayford, Eric R. Kandel, Susumu Tonegawa

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830 Scopus citations

Abstract

Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Original languageEnglish (US)
Pages (from-to)1317-1326
Number of pages10
JournalCell
Volume87
Issue number7
DOIs
StatePublished - Dec 27 1996

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tsien, J. Z., Chen, D. F., Gerber, D., Tom, C., Mercer, E. H., Anderson, D. J., Mayford, M., Kandel, E. R., & Tonegawa, S. (1996). Subregion- and cell type-restricted gene knockout in mouse brain. Cell, 87(7), 1317-1326. https://doi.org/10.1016/S0092-8674(00)81826-7