Subregion- and cell type-restricted gene knockout in mouse brain

Joe Z. Tsien; Dong Feng Chen; David Gerber; Cindy Tom; Eric H. Mercer; David J. Anderson; Mark Mayford; Eric R. Kandel; Susumu Tonegawa

doi:10.1016/S0092-8674(00)81826-7

Subregion- and cell type-restricted gene knockout in mouse brain

Joe Z. Tsien, Dong Feng Chen, David Gerber, Cindy Tom, Eric H. Mercer, David J. Anderson, Mark Mayford, Eric R. Kandel, Susumu Tonegawa

Research output: Contribution to journal › Article › peer-review

1002 Scopus citations

Abstract

Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Original language	English (US)
Pages (from-to)	1317-1326
Number of pages	10
Journal	Cell
Volume	87
Issue number	7
DOIs	https://doi.org/10.1016/S0092-8674(00)81826-7
State	Published - Dec 27 1996
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/S0092-8674(00)81826-7

Cite this

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title = "Subregion- and cell type-restricted gene knockout in mouse brain",

abstract = "Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.",

author = "Tsien, {Joe Z.} and Chen, {Dong Feng} and David Gerber and Cindy Tom and Mercer, {Eric H.} and Anderson, {David J.} and Mark Mayford and Kandel, {Eric R.} and Susumu Tonegawa",

note = "Funding Information: We wish to thank Jason Derwin for technical help and Emily Rossie and Pamela Woronoff for secretarial help. We are grateful to Brian Sauer for the gift of plasmid pBS317 and to Nobuki Nakanishi for the gift of plasmid pNN265. This work was supported by National Institutes of Health grant NS32925 and by gifts from the Shionogi Institute for Medical Science and Amgen, Inc. (all to S.T.). ",

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doi = "10.1016/S0092-8674(00)81826-7",

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T1 - Subregion- and cell type-restricted gene knockout in mouse brain

AU - Tsien, Joe Z.

AU - Chen, Dong Feng

AU - Gerber, David

AU - Tom, Cindy

AU - Mercer, Eric H.

AU - Anderson, David J.

AU - Mayford, Mark

AU - Kandel, Eric R.

AU - Tonegawa, Susumu

N1 - Funding Information: We wish to thank Jason Derwin for technical help and Emily Rossie and Pamela Woronoff for secretarial help. We are grateful to Brian Sauer for the gift of plasmid pBS317 and to Nobuki Nakanishi for the gift of plasmid pNN265. This work was supported by National Institutes of Health grant NS32925 and by gifts from the Shionogi Institute for Medical Science and Amgen, Inc. (all to S.T.).

PY - 1996/12/27

Y1 - 1996/12/27

N2 - Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

AB - Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

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JO - Cell

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Subregion- and cell type-restricted gene knockout in mouse brain

Abstract

ASJC Scopus subject areas

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