Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time

A. Mazzoni, V. Angeloni, N. Sartori, S. Duarte, T. Maravic, L. Tjäderhane, David Henry Pashley, Franklin Chi Meng Tay, L. Breschi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) has recently been investigated for its effectiveness in the prevention of collagen degradation over time and the improvement of resin-dentin bond durability. The objective of the present study was to evaluate the effects of a 0.3 M EDC-containing conditioner on endogenous enzymatic activities within the hybrid layer (HL) created by a self-etch or an etch-and-rinse adhesive after 1 y. The activity within the HL was examined using in situ zymography and confocal laser scanning microscopy after 24 h or 1-y storage in artificial saliva. Dentin specimens were bonded with Clearfil SE Bond (CSE) or XP Bond (XPB). For CSE, the self-etching primer was applied and treated with 0.3 M EDC for 1 min, and then the bonding agent was applied. For XPB, dentin was etched and treated with 0.3 M EDC for 1 min and then bonded with the primer-bonding agent. Control specimens were prepared without EDC treatment. Slices containing the adhesive-dentin interface were covered with fluorescein-conjugated gelatin and observed with a multiphoton confocal microscope. Fluorescence intensity emitted by hydrolyzed fluorescein-conjugated gelatin was quantified, and the amount of gelatinolytic activity was represented by the percentage of green fluorescence emitted within the HL. After 24 h of storage, enzymatic activity was detected by in situ zymography within the HLs of both tested adhesives, with XPB higher than CSE (P < 0.05). Almost no fluorescence signal was detected when specimens were pretreated with EDC compared to controls (P < 0.05). After 1 y of storage, enzymatic activities significantly increased for all groups (excluding XPB control) compared to 24-h storage (P < 0.05), with EDC pretreated specimens exhibiting significantly lower activity than controls (P < 0.05). The present study showed, for the first time, that the use of EDC for both the self-etch and the etch-and-rinse approaches results in the reduction but not complete inhibition of matrix-bound collagenolytic enzyme activities over time in the HL.

Original languageEnglish (US)
Pages (from-to)902-908
Number of pages7
JournalJournal of Dental Research
Volume96
Issue number8
DOIs
StatePublished - Jul 1 2017

Fingerprint

Carbodiimides
Dentin
Adhesives
Fluorescence
Enzymes
Gelatin
Fluorescein
Ethyldimethylaminopropyl Carbodiimide
Artificial Saliva
Confocal Microscopy
Collagen
XP-Bond
Clearfil SE Bond

Keywords

  • adhesives
  • collagen(s)
  • dentin
  • enzymology
  • matrix metalloproteinases
  • microscopy

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mazzoni, A., Angeloni, V., Sartori, N., Duarte, S., Maravic, T., Tjäderhane, L., ... Breschi, L. (2017). Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time. Journal of Dental Research, 96(8), 902-908. https://doi.org/10.1177/0022034517708312

Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time. / Mazzoni, A.; Angeloni, V.; Sartori, N.; Duarte, S.; Maravic, T.; Tjäderhane, L.; Pashley, David Henry; Tay, Franklin Chi Meng; Breschi, L.

In: Journal of Dental Research, Vol. 96, No. 8, 01.07.2017, p. 902-908.

Research output: Contribution to journalArticle

Mazzoni, A, Angeloni, V, Sartori, N, Duarte, S, Maravic, T, Tjäderhane, L, Pashley, DH, Tay, FCM & Breschi, L 2017, 'Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time', Journal of Dental Research, vol. 96, no. 8, pp. 902-908. https://doi.org/10.1177/0022034517708312
Mazzoni A, Angeloni V, Sartori N, Duarte S, Maravic T, Tjäderhane L et al. Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time. Journal of Dental Research. 2017 Jul 1;96(8):902-908. https://doi.org/10.1177/0022034517708312
Mazzoni, A. ; Angeloni, V. ; Sartori, N. ; Duarte, S. ; Maravic, T. ; Tjäderhane, L. ; Pashley, David Henry ; Tay, Franklin Chi Meng ; Breschi, L. / Substantivity of Carbodiimide Inhibition on Dentinal Enzyme Activity over Time. In: Journal of Dental Research. 2017 ; Vol. 96, No. 8. pp. 902-908.
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