Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs

Adrian E. Platts, David J. Dix, Hector E. Chemes, Kary E. Thompson, Robert Goodrich, John C. Rockett, Vanesa Y. Rawe, Silvina Quintana, Michael P. Diamond, Lillian F. Strader, Stephen A. Krawetz

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although the role of some of the structures and proteins as requisite elements for early human development has been established, the function of the sperm-delivered RNAs remains a point for discussion. The presence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that precedes late spermiogenesis. A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals. Unsupervised clustering of the data followed by pathway and ontological analysis revealed the transcriptional perturbation common to the affected individuals. Transcripts encoding components of various cellular remodeling pathways, such as the ubiquitin - proteosome pathway, were severely disrupted. The origin of the perturbation could be traced as far back as the pachytene stage of spermatogenesis. It is anticipated that this diagnostic strategy will prove valuable for understanding male factor infertility.

Original languageEnglish (US)
Pages (from-to)763-773
Number of pages11
JournalHuman Molecular Genetics
Volume16
Issue number7
DOIs
StatePublished - Apr 1 2007

Fingerprint

Spermatogenesis
Spermatozoa
RNA
Pachytene Stage
Male Infertility
Human Development
Ubiquitin
Fertilization
Cluster Analysis
Proteins
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Platts, A. E., Dix, D. J., Chemes, H. E., Thompson, K. E., Goodrich, R., Rockett, J. C., ... Krawetz, S. A. (2007). Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs. Human Molecular Genetics, 16(7), 763-773. https://doi.org/10.1093/hmg/ddm012

Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs. / Platts, Adrian E.; Dix, David J.; Chemes, Hector E.; Thompson, Kary E.; Goodrich, Robert; Rockett, John C.; Rawe, Vanesa Y.; Quintana, Silvina; Diamond, Michael P.; Strader, Lillian F.; Krawetz, Stephen A.

In: Human Molecular Genetics, Vol. 16, No. 7, 01.04.2007, p. 763-773.

Research output: Contribution to journalArticle

Platts, AE, Dix, DJ, Chemes, HE, Thompson, KE, Goodrich, R, Rockett, JC, Rawe, VY, Quintana, S, Diamond, MP, Strader, LF & Krawetz, SA 2007, 'Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs', Human Molecular Genetics, vol. 16, no. 7, pp. 763-773. https://doi.org/10.1093/hmg/ddm012
Platts AE, Dix DJ, Chemes HE, Thompson KE, Goodrich R, Rockett JC et al. Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs. Human Molecular Genetics. 2007 Apr 1;16(7):763-773. https://doi.org/10.1093/hmg/ddm012
Platts, Adrian E. ; Dix, David J. ; Chemes, Hector E. ; Thompson, Kary E. ; Goodrich, Robert ; Rockett, John C. ; Rawe, Vanesa Y. ; Quintana, Silvina ; Diamond, Michael P. ; Strader, Lillian F. ; Krawetz, Stephen A. / Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs. In: Human Molecular Genetics. 2007 ; Vol. 16, No. 7. pp. 763-773.
@article{decfc578dd5949ce86deef8951168c7a,
title = "Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs",
abstract = "We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although the role of some of the structures and proteins as requisite elements for early human development has been established, the function of the sperm-delivered RNAs remains a point for discussion. The presence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that precedes late spermiogenesis. A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals. Unsupervised clustering of the data followed by pathway and ontological analysis revealed the transcriptional perturbation common to the affected individuals. Transcripts encoding components of various cellular remodeling pathways, such as the ubiquitin - proteosome pathway, were severely disrupted. The origin of the perturbation could be traced as far back as the pachytene stage of spermatogenesis. It is anticipated that this diagnostic strategy will prove valuable for understanding male factor infertility.",
author = "Platts, {Adrian E.} and Dix, {David J.} and Chemes, {Hector E.} and Thompson, {Kary E.} and Robert Goodrich and Rockett, {John C.} and Rawe, {Vanesa Y.} and Silvina Quintana and Diamond, {Michael P.} and Strader, {Lillian F.} and Krawetz, {Stephen A.}",
year = "2007",
month = "4",
day = "1",
doi = "10.1093/hmg/ddm012",
language = "English (US)",
volume = "16",
pages = "763--773",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs

AU - Platts, Adrian E.

AU - Dix, David J.

AU - Chemes, Hector E.

AU - Thompson, Kary E.

AU - Goodrich, Robert

AU - Rockett, John C.

AU - Rawe, Vanesa Y.

AU - Quintana, Silvina

AU - Diamond, Michael P.

AU - Strader, Lillian F.

AU - Krawetz, Stephen A.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although the role of some of the structures and proteins as requisite elements for early human development has been established, the function of the sperm-delivered RNAs remains a point for discussion. The presence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that precedes late spermiogenesis. A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals. Unsupervised clustering of the data followed by pathway and ontological analysis revealed the transcriptional perturbation common to the affected individuals. Transcripts encoding components of various cellular remodeling pathways, such as the ubiquitin - proteosome pathway, were severely disrupted. The origin of the perturbation could be traced as far back as the pachytene stage of spermatogenesis. It is anticipated that this diagnostic strategy will prove valuable for understanding male factor infertility.

AB - We are coming to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suite of structures, proteins and RNAs. Although the role of some of the structures and proteins as requisite elements for early human development has been established, the function of the sperm-delivered RNAs remains a point for discussion. The presence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that precedes late spermiogenesis. A cross-platform microarray strategy was used to assess the profile of human spermatozoal transcripts from fertile males who had fathered at least one child compared to teratozoospermic individuals. Unsupervised clustering of the data followed by pathway and ontological analysis revealed the transcriptional perturbation common to the affected individuals. Transcripts encoding components of various cellular remodeling pathways, such as the ubiquitin - proteosome pathway, were severely disrupted. The origin of the perturbation could be traced as far back as the pachytene stage of spermatogenesis. It is anticipated that this diagnostic strategy will prove valuable for understanding male factor infertility.

UR - http://www.scopus.com/inward/record.url?scp=34447323875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447323875&partnerID=8YFLogxK

U2 - 10.1093/hmg/ddm012

DO - 10.1093/hmg/ddm012

M3 - Article

C2 - 17327269

AN - SCOPUS:34447323875

VL - 16

SP - 763

EP - 773

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

IS - 7

ER -