Sulfated Glucuronyl Paragloboside in Rat Brain Microvessels

Nobuyuki Miyatani, Tatsuo Kohriyama, Yasuhiro Maeda, Robert K Yu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In patients with neuropathy associated with para‐proteinemia, there are monoclonal immunoglobulin M antibodies reacting with myelin‐associated glycoprotein and sulfated glucuronyl glycolipids. There are indications that the monoclonal antibodies may be responsible for these neuropathies. However, the mechanism by which the antibodies gain access to the nervous tissue, which is separated by the blood‐brain barrier or blood‐nerve barrier, is still unknown. In this study, we examined the presence of the sulfated glucuronyl glycolipid antigens on brain endothelial cells. Micro‐vessels were isolated from adult Lewis rat brain cortex. Sulfated glucuronyl paragloboside (SGPG) was detected in the acidic lipid fraction by a TLC immunostaining method. Immunofluorescence studies showed positive staining on the surface of microvessels. In addition, SGPG could be detected in the cultured endothelial cells of human umbilical vein. These findings suggest that the endothelial cells contain an‐tigenic sites for interaction with the autoantibodies. This type of interaction may result in damages to the endothelial cell function and may be responsible for changes in the blood‐brain barrier permeability and the ensuing penetration of large molecules, such as immunoglobulins, into the endo‐neurial space.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalJournal of Neurochemistry
Volume55
Issue number2
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Endothelial cells
Microvessels
Rats
Brain
Endothelial Cells
Glycolipids
Nerve Tissue
Umbilical Veins
Paraproteinemias
Antibodies
Autoantibodies
Fluorescent Antibody Technique
Immunoglobulin M
Immunoglobulins
Cultured Cells
Permeability
Glycoproteins
Monoclonal Antibodies
Tissue
Staining and Labeling

Keywords

  • Blood‐brain barrier
  • Blood‐nerve barrier
  • Endothelial cells
  • Neuropathy.
  • Sulfated glucuronyl glycolipids

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Sulfated Glucuronyl Paragloboside in Rat Brain Microvessels. / Miyatani, Nobuyuki; Kohriyama, Tatsuo; Maeda, Yasuhiro; Yu, Robert K.

In: Journal of Neurochemistry, Vol. 55, No. 2, 01.01.1990, p. 577-582.

Research output: Contribution to journalArticle

Miyatani, Nobuyuki ; Kohriyama, Tatsuo ; Maeda, Yasuhiro ; Yu, Robert K. / Sulfated Glucuronyl Paragloboside in Rat Brain Microvessels. In: Journal of Neurochemistry. 1990 ; Vol. 55, No. 2. pp. 577-582.
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