SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses

Ping Yang, Yushan Zhang, Junfa Xu, Shu Zhang, Qilin Yu, Junfeng Pang, Xiaoquan Rao, Michal Kuczma, Mario B. Marrero, David J Fulton, Piotr Kraj, Yunchao Su, Cong Yi Wang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

As a versatile regulatory mechanism, sumoylation has been found to be essential for ordered diverse cellular processes. However, the exact impact of sumoylation on endothelial function largely remained elusive. Here we investigated the role of small ubiquitin-like modifier 1 (SUMO1) mediated sumoylation in the regulation of endothelial function by examining its effect on angiogenesis and homeostatic responses. Adenoviral-mediated SUMO1 expression in porcine aortic endothelial cells (PAECs) dose-dependently promoted proliferation, migration and tube formation. In line with these results in PAECs, Matrigel plug assays in SUMO1 transgenic mice demonstrated a significant higher capacity for vascular neogenesis as compared with that of control littermates. Moreover, SUMO1 expression protected PAECs from serum starvation or H2O2-induced apoptosis. Mechanistic studies demonstrated that SUMO1 sumoylation modulates ERK1/2 activation and MMP13 expression as well as Jak2/STAT5 signaling to promote angiogenesis. SUMO1 sumoylation also suppressed NFκB and c-JUN transcriptional activity to provide protection for PAECs against oxidative stress-induced apoptosis. Given that sumoylation is a reversible process, dynamic regulation of the sumoylation function could be a novel strategy to modulate endothelial function in disease states.

Original languageEnglish (US)
Pages (from-to)427-440
Number of pages14
JournalAmerican Journal of Translational Research
Volume5
Issue number4
StatePublished - Jan 1 2013

Fingerprint

Sumoylation
Ubiquitin
Endothelial cells
Swine
Endothelial Cells
Apoptosis
Oxidative stress
Starvation
Assays
Transgenic Mice
Blood Vessels
Chemical activation
Oxidative Stress
Serum

Keywords

  • Angiogenesis
  • Endothelial cells
  • SUMO1
  • Sumoylation

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Cancer Research

Cite this

SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses. / Yang, Ping; Zhang, Yushan; Xu, Junfa; Zhang, Shu; Yu, Qilin; Pang, Junfeng; Rao, Xiaoquan; Kuczma, Michal; Marrero, Mario B.; Fulton, David J; Kraj, Piotr; Su, Yunchao; Wang, Cong Yi.

In: American Journal of Translational Research, Vol. 5, No. 4, 01.01.2013, p. 427-440.

Research output: Contribution to journalArticle

Yang, P, Zhang, Y, Xu, J, Zhang, S, Yu, Q, Pang, J, Rao, X, Kuczma, M, Marrero, MB, Fulton, DJ, Kraj, P, Su, Y & Wang, CY 2013, 'SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses', American Journal of Translational Research, vol. 5, no. 4, pp. 427-440.
Yang, Ping ; Zhang, Yushan ; Xu, Junfa ; Zhang, Shu ; Yu, Qilin ; Pang, Junfeng ; Rao, Xiaoquan ; Kuczma, Michal ; Marrero, Mario B. ; Fulton, David J ; Kraj, Piotr ; Su, Yunchao ; Wang, Cong Yi. / SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses. In: American Journal of Translational Research. 2013 ; Vol. 5, No. 4. pp. 427-440.
@article{0be8a694707347929c77399937003396,
title = "SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses",
abstract = "As a versatile regulatory mechanism, sumoylation has been found to be essential for ordered diverse cellular processes. However, the exact impact of sumoylation on endothelial function largely remained elusive. Here we investigated the role of small ubiquitin-like modifier 1 (SUMO1) mediated sumoylation in the regulation of endothelial function by examining its effect on angiogenesis and homeostatic responses. Adenoviral-mediated SUMO1 expression in porcine aortic endothelial cells (PAECs) dose-dependently promoted proliferation, migration and tube formation. In line with these results in PAECs, Matrigel plug assays in SUMO1 transgenic mice demonstrated a significant higher capacity for vascular neogenesis as compared with that of control littermates. Moreover, SUMO1 expression protected PAECs from serum starvation or H2O2-induced apoptosis. Mechanistic studies demonstrated that SUMO1 sumoylation modulates ERK1/2 activation and MMP13 expression as well as Jak2/STAT5 signaling to promote angiogenesis. SUMO1 sumoylation also suppressed NFκB and c-JUN transcriptional activity to provide protection for PAECs against oxidative stress-induced apoptosis. Given that sumoylation is a reversible process, dynamic regulation of the sumoylation function could be a novel strategy to modulate endothelial function in disease states.",
keywords = "Angiogenesis, Endothelial cells, SUMO1, Sumoylation",
author = "Ping Yang and Yushan Zhang and Junfa Xu and Shu Zhang and Qilin Yu and Junfeng Pang and Xiaoquan Rao and Michal Kuczma and Marrero, {Mario B.} and Fulton, {David J} and Piotr Kraj and Yunchao Su and Wang, {Cong Yi}",
year = "2013",
month = "1",
day = "1",
language = "English (US)",
volume = "5",
pages = "427--440",
journal = "American Journal of Translational Research",
issn = "1943-8141",
publisher = "e-Century Publishing Corporation",
number = "4",

}

TY - JOUR

T1 - SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses

AU - Yang, Ping

AU - Zhang, Yushan

AU - Xu, Junfa

AU - Zhang, Shu

AU - Yu, Qilin

AU - Pang, Junfeng

AU - Rao, Xiaoquan

AU - Kuczma, Michal

AU - Marrero, Mario B.

AU - Fulton, David J

AU - Kraj, Piotr

AU - Su, Yunchao

AU - Wang, Cong Yi

PY - 2013/1/1

Y1 - 2013/1/1

N2 - As a versatile regulatory mechanism, sumoylation has been found to be essential for ordered diverse cellular processes. However, the exact impact of sumoylation on endothelial function largely remained elusive. Here we investigated the role of small ubiquitin-like modifier 1 (SUMO1) mediated sumoylation in the regulation of endothelial function by examining its effect on angiogenesis and homeostatic responses. Adenoviral-mediated SUMO1 expression in porcine aortic endothelial cells (PAECs) dose-dependently promoted proliferation, migration and tube formation. In line with these results in PAECs, Matrigel plug assays in SUMO1 transgenic mice demonstrated a significant higher capacity for vascular neogenesis as compared with that of control littermates. Moreover, SUMO1 expression protected PAECs from serum starvation or H2O2-induced apoptosis. Mechanistic studies demonstrated that SUMO1 sumoylation modulates ERK1/2 activation and MMP13 expression as well as Jak2/STAT5 signaling to promote angiogenesis. SUMO1 sumoylation also suppressed NFκB and c-JUN transcriptional activity to provide protection for PAECs against oxidative stress-induced apoptosis. Given that sumoylation is a reversible process, dynamic regulation of the sumoylation function could be a novel strategy to modulate endothelial function in disease states.

AB - As a versatile regulatory mechanism, sumoylation has been found to be essential for ordered diverse cellular processes. However, the exact impact of sumoylation on endothelial function largely remained elusive. Here we investigated the role of small ubiquitin-like modifier 1 (SUMO1) mediated sumoylation in the regulation of endothelial function by examining its effect on angiogenesis and homeostatic responses. Adenoviral-mediated SUMO1 expression in porcine aortic endothelial cells (PAECs) dose-dependently promoted proliferation, migration and tube formation. In line with these results in PAECs, Matrigel plug assays in SUMO1 transgenic mice demonstrated a significant higher capacity for vascular neogenesis as compared with that of control littermates. Moreover, SUMO1 expression protected PAECs from serum starvation or H2O2-induced apoptosis. Mechanistic studies demonstrated that SUMO1 sumoylation modulates ERK1/2 activation and MMP13 expression as well as Jak2/STAT5 signaling to promote angiogenesis. SUMO1 sumoylation also suppressed NFκB and c-JUN transcriptional activity to provide protection for PAECs against oxidative stress-induced apoptosis. Given that sumoylation is a reversible process, dynamic regulation of the sumoylation function could be a novel strategy to modulate endothelial function in disease states.

KW - Angiogenesis

KW - Endothelial cells

KW - SUMO1

KW - Sumoylation

UR - http://www.scopus.com/inward/record.url?scp=84878312204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878312204&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 427

EP - 440

JO - American Journal of Translational Research

JF - American Journal of Translational Research

SN - 1943-8141

IS - 4

ER -