Suppression of T-cell chemokines by Porphyromonas gingivalis

Catherine Ella Jauregui, Qian Wang, Christopher J. Wright, Hiroki Takeuchi, Silvia M. Uriarte, Richard J. Lamont

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. In this study, we found that P. gingivalis did not induce the expression of the T-cell chemokine IP-10 (CXCL10) from neutrophils, peripheral blood mononuclear cells (PBMCs), or gingival epithelial cells. Furthermore, P. gingivalis suppressed gamma interferon (IFN-γ)-stimulated release of IP-10, ITAC (CXCL11), and Mig (CXCL9) from epithelial cells and inhibited IP-10 secretion in a mixed infection with the otherwise stimulatory Fusobacterium nucleatum. Inhibition of chemokine expression occurred at the level of gene transcription and was associated with downregulation of interferon regulatory factor 1 (IRF-1) and decreased levels of Stat1. Ectopic expression of IRF-1 in epithelial cells relieved P. gingivalis-induced inhibition of IP-10 release. Direct contact between P. gingivalis and epithelial cells was not required for IP-10 inhibition. These results highlight the immune-disruptive potential of P. gingivalis. Suppression of IP-10 and other Th1-biasing chemokines by P. gingivalis may perturb the balance of protective and destructive immunity in the periodontal tissues and facilitate the pathogenicity of oral microbial communities.

Original languageEnglish (US)
Pages (from-to)2288-2295
Number of pages8
JournalInfection and Immunity
Volume81
Issue number7
DOIs
StatePublished - Jul 1 2013

Fingerprint

Porphyromonas gingivalis
Chemokines
T-Lymphocytes
Epithelial Cells
Interferon Regulatory Factor-1
Dysbiosis
Fusobacterium nucleatum
Periodontal Diseases
Coinfection
Interferon-gamma
Virulence
Immunity
Blood Cells
Neutrophils
Down-Regulation
Genes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Jauregui, C. E., Wang, Q., Wright, C. J., Takeuchi, H., Uriarte, S. M., & Lamont, R. J. (2013). Suppression of T-cell chemokines by Porphyromonas gingivalis. Infection and Immunity, 81(7), 2288-2295. https://doi.org/10.1128/IAI.00264-13

Suppression of T-cell chemokines by Porphyromonas gingivalis. / Jauregui, Catherine Ella; Wang, Qian; Wright, Christopher J.; Takeuchi, Hiroki; Uriarte, Silvia M.; Lamont, Richard J.

In: Infection and Immunity, Vol. 81, No. 7, 01.07.2013, p. 2288-2295.

Research output: Contribution to journalArticle

Jauregui, CE, Wang, Q, Wright, CJ, Takeuchi, H, Uriarte, SM & Lamont, RJ 2013, 'Suppression of T-cell chemokines by Porphyromonas gingivalis', Infection and Immunity, vol. 81, no. 7, pp. 2288-2295. https://doi.org/10.1128/IAI.00264-13
Jauregui CE, Wang Q, Wright CJ, Takeuchi H, Uriarte SM, Lamont RJ. Suppression of T-cell chemokines by Porphyromonas gingivalis. Infection and Immunity. 2013 Jul 1;81(7):2288-2295. https://doi.org/10.1128/IAI.00264-13
Jauregui, Catherine Ella ; Wang, Qian ; Wright, Christopher J. ; Takeuchi, Hiroki ; Uriarte, Silvia M. ; Lamont, Richard J. / Suppression of T-cell chemokines by Porphyromonas gingivalis. In: Infection and Immunity. 2013 ; Vol. 81, No. 7. pp. 2288-2295.
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