Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin

Natsumi Fujiwara, Keiji Murakami, Kaya Yoshida, Shunsuke Sakurai, Yasusei Kudo, Kazumi Ozaki, Katsuhiko Hirota, Hideki Fujii, Maiko Suzuki, Yoichiro Miyake, Hiromichi Yumoto

Research output: Contribution to journalArticle

Abstract

Objectives: The effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of microorganisms such as non-Candida albicans Candida (NCAC) and methicillin-resistant Staphylococcus aureus (MRSA), frequently detected in oral infections in immunocompromised and/or elderly people, to denture resin material, are still unclear. Here, we report the effects of MPC-polymer on the adherence of C. albicans, NCAC, and MRSA to acrylic denture resin. Methods: Sixteen strains of C. albicans, seven strains of C. glabrata, two strains of C. tropicalis, one strain of C. parapsilosis, and six strains of MRSA were used. We cultured the fungal/bacterial strains and examined the cell growth and adherence of fungi/bacteria to mucin-coated acrylic denture resin plates (ADRP) with or without MPC-polymer coating, by scanning electron microscopy. The cell surface hydrophobicity of the fungal/bacterial strains was measured by the adsorption to hydrocarbons. Results: MPC-polymer did not affect the growth of all strains of Candida species and MRSA, but significantly suppressed adherence to ADRP in most strains of C. albicans and all strains of NCAC and MRSA. A significant positive correlation was found between cell hydrophobicity and the reduction rates of microbial adherence to ADRP treated with 5% of MPC-polymer. Conclusions: MPC-polymer treatment for acrylic resin material suppresses the adherence of C. albicans, NCAC and MRSA via their hydrophilicity interaction. Clinical significance: The application of MPC-polymer for denture hygiene is potent to prevent oral candidiasis, denture stomatitis and opportunistic infection, caused by Candida species and MRSA, via suppressing the adherence of those fungus/bacteria.

Original languageEnglish (US)
Article numbere04211
JournalHeliyon
Volume6
Issue number6
DOIs
StatePublished - Jun 2020

Keywords

  • 2-Methacryloyloxyethyl phosphorylcholine
  • Candida
  • Dental materials
  • Dentistry
  • Denture
  • Hydrophilicity
  • MRSA
  • Medical microbiology
  • Microbiology
  • Oral medicine
  • Prosthetic dentistry

ASJC Scopus subject areas

  • General

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  • Cite this

    Fujiwara, N., Murakami, K., Yoshida, K., Sakurai, S., Kudo, Y., Ozaki, K., Hirota, K., Fujii, H., Suzuki, M., Miyake, Y., & Yumoto, H. (2020). Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin. Heliyon, 6(6), [e04211]. https://doi.org/10.1016/j.heliyon.2020.e04211