Suppressive effects of edible Thai plants on superoxide and nitric oxide generation

Suratwadee Jiwajinda, Vilai Santisopasri, Akira Murakami, Oe Kyung Kim, Ha Won Kim, Hajime Ohigashi

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We screened ethanol extracts from a total of 134 species of edible Thai plants for their suppressive effects on superoxide (O2-) generation using a xanthine (XA)-xanthine oxidase (XOD) assay system. When the extracts were tested at a concentration of 500 μg/ml, 28.4% significantly suppressed O2- generation. Of these active extracts, it was found that in 17.9% of cases the action was due to XOD inhibition, in 1.5% due to O2- scavenging activity, and in 9% due to both XOD inhibition and O2- scavenging. In addition, some plant extracts (25 species) which had been known to possibly possess anti-tumor promoting activity were tested for O2- and NO generation in cellular systems. In this test, 13 species exhibited strong inhibitory activity toward both O2- and NO generation. From the fruit pods of Oroxylum indicum (Bignoniaceae), a traditional vegetable in Thailand, two flavones, oroxylin A and chrysin, and a triterpene carboxylic acid, ursolic acid (UA), were identified as inhibitors of O2- generation in XA/XOD system. These compounds also showed marked inhibitory effects on the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced O2- generation in dimethylsulfoxide (DMSO)-differentiated HL-60 cells. Our results suggest that, as we have reported earlier, edible Thai plants are promising sources of antioxidants with chemopreventive potential.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalAsian Pacific Journal of Cancer Prevention
Volume3
Issue number3
StatePublished - 2002
Externally publishedYes

Fingerprint

Edible Plants
Xanthine Oxidase
Superoxides
Nitric Oxide
Xanthine
Bignoniaceae
Flavones
Triterpenes
HL-60 Cells
Plant Extracts
Tetradecanoylphorbol Acetate
Thailand
Carboxylic Acids
Dimethyl Sulfoxide
Vegetables
Carcinogens
Fruit
Ethanol
Antioxidants
Neoplasms

Keywords

  • Anti-oxidation
  • Chrysin
  • Oroxylin A
  • Oroxylum indicum
  • Thai plants
  • Ursolic acid

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Jiwajinda, S., Santisopasri, V., Murakami, A., Kim, O. K., Kim, H. W., & Ohigashi, H. (2002). Suppressive effects of edible Thai plants on superoxide and nitric oxide generation. Asian Pacific Journal of Cancer Prevention, 3(3), 215-223.

Suppressive effects of edible Thai plants on superoxide and nitric oxide generation. / Jiwajinda, Suratwadee; Santisopasri, Vilai; Murakami, Akira; Kim, Oe Kyung; Kim, Ha Won; Ohigashi, Hajime.

In: Asian Pacific Journal of Cancer Prevention, Vol. 3, No. 3, 2002, p. 215-223.

Research output: Contribution to journalArticle

Jiwajinda, S, Santisopasri, V, Murakami, A, Kim, OK, Kim, HW & Ohigashi, H 2002, 'Suppressive effects of edible Thai plants on superoxide and nitric oxide generation', Asian Pacific Journal of Cancer Prevention, vol. 3, no. 3, pp. 215-223.
Jiwajinda, Suratwadee ; Santisopasri, Vilai ; Murakami, Akira ; Kim, Oe Kyung ; Kim, Ha Won ; Ohigashi, Hajime. / Suppressive effects of edible Thai plants on superoxide and nitric oxide generation. In: Asian Pacific Journal of Cancer Prevention. 2002 ; Vol. 3, No. 3. pp. 215-223.
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