Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages

Ha Won Kim, Akira Murakami, Masako Abe, Yoshio Ozawa, Yasujiro Morimitsu, Marshall V. Williams, Hajime Ohigashi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O2 -) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities. In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 μM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O2 - generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-γ- induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs] = 84.6% and 95.9%, respectively). Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR = 95.9%). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs = 99.1%, 74.6%, 74.0%, and 64.4%, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor κB was suppressed by this compound (IR = 100%), suggesting that the suppression of nuclear factor κB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammation-associated carcinogenesis.

Original languageEnglish (US)
Pages (from-to)1621-1629
Number of pages9
JournalAntioxidants and Redox Signaling
Volume7
Issue number11-12
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Ginger
Reactive Nitrogen Species
Macrophages
Reactive Oxygen Species
Genes
HL-60 Cells
Lipopolysaccharides
Edible Plants
Xanthine Oxidase
NADPH Oxidase
Corrosion inhibitors
Tetradecanoylphorbol Acetate
Nitric Oxide Synthase Type II
Granulocyte-Macrophage Colony-Stimulating Factor
Chloroform
Interleukin-1
Gene expression
Superoxides
Interferons
Free Radicals

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages. / Kim, Ha Won; Murakami, Akira; Abe, Masako; Ozawa, Yoshio; Morimitsu, Yasujiro; Williams, Marshall V.; Ohigashi, Hajime.

In: Antioxidants and Redox Signaling, Vol. 7, No. 11-12, 01.11.2005, p. 1621-1629.

Research output: Contribution to journalArticle

Kim, Ha Won ; Murakami, Akira ; Abe, Masako ; Ozawa, Yoshio ; Morimitsu, Yasujiro ; Williams, Marshall V. ; Ohigashi, Hajime. / Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages. In: Antioxidants and Redox Signaling. 2005 ; Vol. 7, No. 11-12. pp. 1621-1629.
@article{f5884c4cd5e6400d99c26ced284f4fe0,
title = "Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages",
abstract = "We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O2 -) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities. In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 μM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O2 - generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-γ- induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs] = 84.6{\%} and 95.9{\%}, respectively). Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR = 95.9{\%}). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs = 99.1{\%}, 74.6{\%}, 74.0{\%}, and 64.4{\%}, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor κB was suppressed by this compound (IR = 100{\%}), suggesting that the suppression of nuclear factor κB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammation-associated carcinogenesis.",
author = "Kim, {Ha Won} and Akira Murakami and Masako Abe and Yoshio Ozawa and Yasujiro Morimitsu and Williams, {Marshall V.} and Hajime Ohigashi",
year = "2005",
month = "11",
day = "1",
doi = "10.1089/ars.2005.7.1621",
language = "English (US)",
volume = "7",
pages = "1621--1629",
journal = "Antioxidants and Redox Signaling",
issn = "1523-0864",
publisher = "Mary Ann Liebert Inc.",
number = "11-12",

}

TY - JOUR

T1 - Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages

AU - Kim, Ha Won

AU - Murakami, Akira

AU - Abe, Masako

AU - Ozawa, Yoshio

AU - Morimitsu, Yasujiro

AU - Williams, Marshall V.

AU - Ohigashi, Hajime

PY - 2005/11/1

Y1 - 2005/11/1

N2 - We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O2 -) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities. In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 μM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O2 - generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-γ- induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs] = 84.6% and 95.9%, respectively). Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR = 95.9%). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs = 99.1%, 74.6%, 74.0%, and 64.4%, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor κB was suppressed by this compound (IR = 100%), suggesting that the suppression of nuclear factor κB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammation-associated carcinogenesis.

AB - We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O2 -) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities. In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 μM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O2 - generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-γ- induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs] = 84.6% and 95.9%, respectively). Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR = 95.9%). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1β, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs = 99.1%, 74.6%, 74.0%, and 64.4%, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor κB was suppressed by this compound (IR = 100%), suggesting that the suppression of nuclear factor κB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammation-associated carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=28844485665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28844485665&partnerID=8YFLogxK

U2 - 10.1089/ars.2005.7.1621

DO - 10.1089/ars.2005.7.1621

M3 - Article

C2 - 16356125

AN - SCOPUS:28844485665

VL - 7

SP - 1621

EP - 1629

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1523-0864

IS - 11-12

ER -