Suppressive effects of selected antioxidants on the activated leukocytes-induced mutagenesis in the co-culture assay systems

Ha Won Kim, Akira Murakami, Marshall V. Williams, Hajime Ohigashi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We recently established a novel co-culture assay system using activated inflammatory cells and AS52 Chinese hamster ovary cells, and demonstrated that reactive oxygen and nitrogen species (RONS) generated from activated inflammatory leukocytes induce mutations in the gpt recorder gene in AS52 cells. In this study, we examined the inhibitory effects of 19 agents with antioxidative properties on RONS generation in cultured inflammatory cells and on mutagenesis in AS52 cells co-cultured with activated inflammatory cells. The results demonstrate that there is a linear correlation between the ability of these agents to suppress RONS production in activated inflammatory cells and to inhibit mutation in AS52 cells.

Original languageEnglish (US)
Pages (from-to)238-242
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number1
DOIs
StatePublished - Jan 2004

Keywords

  • AS52
  • HL-60
  • Nitric oxide
  • RAW 264.7
  • Superoxide

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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