Surface treatments on titanium implants via nanostructured ceria for antibacterial and anti-inflammatory capabilities

Xue Li, Manlin Qi, Xiaolin Sun, Michael D. Weir, Franklin Chi Meng Tay, Thomas W. Oates, Biao Dong, Yanmin Zhou, Lin Wang, Hockin H.K. Xu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, the authors developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium (Ti) surfaces to enhance their antibacterial and anti-inflammatory properties. The objectives of the study were to: (1) develop novel Ti surfaces modified with different shapes of nano-CeO2 (nanorod, nanocube and nano-octahedron) for peri-implantitis prevention; (2) investigate and compare the inhibition efficacy of different shapes of CeO2-modified surfaces against biofilms of peri-implantitis-related pathogens; and (3) evaluate the different CeO2-modified surfaces on cell inflammatory response in vitro and in vivo. The results showed that nanorod CeO2-modified Ti had more bacteria attachment of Streptococcus sanguinis in the early stage, compared with other CeO2-modified Ti (p < 0.05). They all exhibited similarly substantial CFU reductions against peri-implantitis-related biofilms (p > 0.1). Nanocube and nano-octahedron CeO2-modified Ti exerted much better anti-inflammatory effects and ROS-scavenging ability than nanorod CeO2 in vitro (p < 0.05). In vivo, the mean mRNA expression of TNF-α, IL-6 and IL-1β in the tissues around Ti was decreased by the three shapes of nano-CeO2; nano-octahedron CeO2 showed the strongest anti-inflammatory effect among all groups (p < 0.05). In conclusion, all three types of CeO2-modified Ti exerted equally strong antibacterial properties; nano-octahedron CeO2-modified Ti had the best anti-inflammatory effect. Therefore, CeO2-modified Ti surfaces are highly promising for enhancing antimicrobial functions for dental implants. Novel nano-octahedron CeO2 coating on Ti had great therapeutic potential for alleviating and eliminating peri-implantitis. Statement of Significance: Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, we developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium surfaces to enhance their antibacterial and anti-inflammatory properties for dental implants. In addition, we found that the nano-octahedron CeO2 coating on titanium would have great therapeutic potential for alleviating and eliminating peri-implantitis.

Original languageEnglish (US)
Pages (from-to)627-643
Number of pages17
JournalActa biomaterialia
Volume94
DOIs
StatePublished - Aug 1 2019

Fingerprint

Cerium compounds
Titanium
Surface treatment
Anti-Inflammatory Agents
Peri-Implantitis
Dental prostheses
Dental Implants
Nanotubes
Nanorods
Coatings
Catalysis
Crystals
Scavenging
Biofilms
Pathogens
Streptococcus
Interleukin-1
Interleukin-6
Bacteria
Tissue

Keywords

  • Anti-inflammation
  • Antibacterial
  • Ceria
  • Peri-implantitis
  • Shape control

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Surface treatments on titanium implants via nanostructured ceria for antibacterial and anti-inflammatory capabilities. / Li, Xue; Qi, Manlin; Sun, Xiaolin; Weir, Michael D.; Tay, Franklin Chi Meng; Oates, Thomas W.; Dong, Biao; Zhou, Yanmin; Wang, Lin; Xu, Hockin H.K.

In: Acta biomaterialia, Vol. 94, 01.08.2019, p. 627-643.

Research output: Contribution to journalArticle

Li, Xue ; Qi, Manlin ; Sun, Xiaolin ; Weir, Michael D. ; Tay, Franklin Chi Meng ; Oates, Thomas W. ; Dong, Biao ; Zhou, Yanmin ; Wang, Lin ; Xu, Hockin H.K. / Surface treatments on titanium implants via nanostructured ceria for antibacterial and anti-inflammatory capabilities. In: Acta biomaterialia. 2019 ; Vol. 94. pp. 627-643.
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abstract = "Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, the authors developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium (Ti) surfaces to enhance their antibacterial and anti-inflammatory properties. The objectives of the study were to: (1) develop novel Ti surfaces modified with different shapes of nano-CeO2 (nanorod, nanocube and nano-octahedron) for peri-implantitis prevention; (2) investigate and compare the inhibition efficacy of different shapes of CeO2-modified surfaces against biofilms of peri-implantitis-related pathogens; and (3) evaluate the different CeO2-modified surfaces on cell inflammatory response in vitro and in vivo. The results showed that nanorod CeO2-modified Ti had more bacteria attachment of Streptococcus sanguinis in the early stage, compared with other CeO2-modified Ti (p < 0.05). They all exhibited similarly substantial CFU reductions against peri-implantitis-related biofilms (p > 0.1). Nanocube and nano-octahedron CeO2-modified Ti exerted much better anti-inflammatory effects and ROS-scavenging ability than nanorod CeO2 in vitro (p < 0.05). In vivo, the mean mRNA expression of TNF-α, IL-6 and IL-1β in the tissues around Ti was decreased by the three shapes of nano-CeO2; nano-octahedron CeO2 showed the strongest anti-inflammatory effect among all groups (p < 0.05). In conclusion, all three types of CeO2-modified Ti exerted equally strong antibacterial properties; nano-octahedron CeO2-modified Ti had the best anti-inflammatory effect. Therefore, CeO2-modified Ti surfaces are highly promising for enhancing antimicrobial functions for dental implants. Novel nano-octahedron CeO2 coating on Ti had great therapeutic potential for alleviating and eliminating peri-implantitis. Statement of Significance: Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, we developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium surfaces to enhance their antibacterial and anti-inflammatory properties for dental implants. In addition, we found that the nano-octahedron CeO2 coating on titanium would have great therapeutic potential for alleviating and eliminating peri-implantitis.",
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AU - Li, Xue

AU - Qi, Manlin

AU - Sun, Xiaolin

AU - Weir, Michael D.

AU - Tay, Franklin Chi Meng

AU - Oates, Thomas W.

AU - Dong, Biao

AU - Zhou, Yanmin

AU - Wang, Lin

AU - Xu, Hockin H.K.

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N2 - Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, the authors developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium (Ti) surfaces to enhance their antibacterial and anti-inflammatory properties. The objectives of the study were to: (1) develop novel Ti surfaces modified with different shapes of nano-CeO2 (nanorod, nanocube and nano-octahedron) for peri-implantitis prevention; (2) investigate and compare the inhibition efficacy of different shapes of CeO2-modified surfaces against biofilms of peri-implantitis-related pathogens; and (3) evaluate the different CeO2-modified surfaces on cell inflammatory response in vitro and in vivo. The results showed that nanorod CeO2-modified Ti had more bacteria attachment of Streptococcus sanguinis in the early stage, compared with other CeO2-modified Ti (p < 0.05). They all exhibited similarly substantial CFU reductions against peri-implantitis-related biofilms (p > 0.1). Nanocube and nano-octahedron CeO2-modified Ti exerted much better anti-inflammatory effects and ROS-scavenging ability than nanorod CeO2 in vitro (p < 0.05). In vivo, the mean mRNA expression of TNF-α, IL-6 and IL-1β in the tissues around Ti was decreased by the three shapes of nano-CeO2; nano-octahedron CeO2 showed the strongest anti-inflammatory effect among all groups (p < 0.05). In conclusion, all three types of CeO2-modified Ti exerted equally strong antibacterial properties; nano-octahedron CeO2-modified Ti had the best anti-inflammatory effect. Therefore, CeO2-modified Ti surfaces are highly promising for enhancing antimicrobial functions for dental implants. Novel nano-octahedron CeO2 coating on Ti had great therapeutic potential for alleviating and eliminating peri-implantitis. Statement of Significance: Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, we developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium surfaces to enhance their antibacterial and anti-inflammatory properties for dental implants. In addition, we found that the nano-octahedron CeO2 coating on titanium would have great therapeutic potential for alleviating and eliminating peri-implantitis.

AB - Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, the authors developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium (Ti) surfaces to enhance their antibacterial and anti-inflammatory properties. The objectives of the study were to: (1) develop novel Ti surfaces modified with different shapes of nano-CeO2 (nanorod, nanocube and nano-octahedron) for peri-implantitis prevention; (2) investigate and compare the inhibition efficacy of different shapes of CeO2-modified surfaces against biofilms of peri-implantitis-related pathogens; and (3) evaluate the different CeO2-modified surfaces on cell inflammatory response in vitro and in vivo. The results showed that nanorod CeO2-modified Ti had more bacteria attachment of Streptococcus sanguinis in the early stage, compared with other CeO2-modified Ti (p < 0.05). They all exhibited similarly substantial CFU reductions against peri-implantitis-related biofilms (p > 0.1). Nanocube and nano-octahedron CeO2-modified Ti exerted much better anti-inflammatory effects and ROS-scavenging ability than nanorod CeO2 in vitro (p < 0.05). In vivo, the mean mRNA expression of TNF-α, IL-6 and IL-1β in the tissues around Ti was decreased by the three shapes of nano-CeO2; nano-octahedron CeO2 showed the strongest anti-inflammatory effect among all groups (p < 0.05). In conclusion, all three types of CeO2-modified Ti exerted equally strong antibacterial properties; nano-octahedron CeO2-modified Ti had the best anti-inflammatory effect. Therefore, CeO2-modified Ti surfaces are highly promising for enhancing antimicrobial functions for dental implants. Novel nano-octahedron CeO2 coating on Ti had great therapeutic potential for alleviating and eliminating peri-implantitis. Statement of Significance: Peri-implantitis is the most common risk factor for dental implant failure. Nanostructured ceria (nano-CeO2) has anti-inflammatory and antibacterial functions, and different shapes of ceria enclosed by specific crystal planes could be an effective approach to enhance intrinsic catalysis. In the present study, we developed a novel implant surface-modification strategy by coating different shapes of nano-CeO2 onto titanium surfaces to enhance their antibacterial and anti-inflammatory properties for dental implants. In addition, we found that the nano-octahedron CeO2 coating on titanium would have great therapeutic potential for alleviating and eliminating peri-implantitis.

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