Surface ultrastructural features of isolated perfused rat hearts during calcium paradox

Muhammad Ashraf, M. Onda, J. Benedict, Y. Hirohata

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cellular damage caused by calcium (Ca++) paradox is of significant importance under both experimental and clinical conditions. Electron microscopy was used to study the surface changes of the isolated rat hearts perfused with normal Krebs-Henseleit (KH) medium, Ca++ -free KH medium, Ca++- free KH medium followed by normal KH medium. The hearts perfused with Ca++ -free medium and normal KH medium showed elongated parallel myofibrils with transverse ridges spaced at regular intervals. The major change in Ca++ -free hearts consisted of wide cellular separation. For the Ca++ -paradox hearts, the myofibrils were twisted and narrowed at several locations. The myocardial fiber surfaces were bulged out, frequently with sarcolemmal ruptures and holes. Supercontraction of cells caused breaks in the sarcolemma and aggregation of mitochondria at the cell periphery. These results suggest that Ca++ -depletion primarily affects the myocardial cell junctions which, following reintroduction of Ca++, accelerate the entry of Ca++ into the cells, supercontraction of cells and stretching of sarcolemma.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalScanning Electron Microscopy
Volume1981
Issue number3
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Rats
Calcium
Sarcolemma
Myofibrils
Mitochondria
Intercellular Junctions
Electron microscopy
Stretching
Rupture
Electron Microscopy
Agglomeration
Fibers

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Biophysics

Cite this

Surface ultrastructural features of isolated perfused rat hearts during calcium paradox. / Ashraf, Muhammad; Onda, M.; Benedict, J.; Hirohata, Y.

In: Scanning Electron Microscopy, Vol. 1981, No. 3, 01.01.1981, p. 109-114.

Research output: Contribution to journalArticle

Ashraf, Muhammad ; Onda, M. ; Benedict, J. ; Hirohata, Y. / Surface ultrastructural features of isolated perfused rat hearts during calcium paradox. In: Scanning Electron Microscopy. 1981 ; Vol. 1981, No. 3. pp. 109-114.
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