Surfactant protein D is expressed and modulates inflammatory responses in human coronary artery smooth muscle cells

Gary D. Snyder, Rebecca E. Oberley-Deegan, Kelli L. Goss, Sara A. Romig-Martin, Lynn L. Stoll, Jeanne M. Snyder, Neal Lee Weintraub

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Surfactant protein D (SP-D) is a constituent of the innate immune system that plays a role in the host defense against lung pathogens and in modulating inflammatory responses. While SP-D has been detected in extrapulmonary tissues, little is known about its expression and function in the vasculature. Immunostaining of human coronary artery tissue sections demonstrated immunoreactive SP-D protein in smooth muscle cells (SMCs) and endothelial cells. SP-D was also detected in isolated human coronary artery SMCs (HCASMCs) by PCR and immunoblot analysis. Treatment of HCASMCs with endotoxin (LPS) stimulated the release of IL-8, a proinflammatory cytokine. This release was inhibited >70% by recombinant SP-D. Overexpression of SP-D by adenoviral-mediated gene transfer in HCASMCs inhibited both LPS-and TNF-α-induced IL-8 release. Overexpression of SP-D also enhanced uptake of Chlamydia pneumoniae elementary bodies into HCASMCs while attenuating IL-8 production induced by bacterial exposure. Both LPS and TNF-α increased SP-D mRNA levels by five-to eightfold in HCASMCs, suggesting that inflammatory mediators upregulate the expression of SP-D. In conclusion, SP-D is expressed in human coronary arteries and functions as an anti-inflammatory protein in HCASMCs. SP-D may also participate in the host defense against pathogens that invade the vascular wall.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume294
Issue number5
DOIs
StatePublished - May 1 2008
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Smooth Muscle Myocytes
Coronary Vessels
Interleukin-8
Chlamydophila pneumoniae
Human Body
Endotoxins
Blood Vessels
Immune System
Proteins
Anti-Inflammatory Agents

Keywords

  • Chlamydia pneumoniae
  • Inflammation
  • Innate immunity
  • Interleukin-8

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Surfactant protein D is expressed and modulates inflammatory responses in human coronary artery smooth muscle cells. / Snyder, Gary D.; Oberley-Deegan, Rebecca E.; Goss, Kelli L.; Romig-Martin, Sara A.; Stoll, Lynn L.; Snyder, Jeanne M.; Weintraub, Neal Lee.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 294, No. 5, 01.05.2008.

Research output: Contribution to journalArticle

Snyder, Gary D. ; Oberley-Deegan, Rebecca E. ; Goss, Kelli L. ; Romig-Martin, Sara A. ; Stoll, Lynn L. ; Snyder, Jeanne M. ; Weintraub, Neal Lee. / Surfactant protein D is expressed and modulates inflammatory responses in human coronary artery smooth muscle cells. In: American Journal of Physiology - Heart and Circulatory Physiology. 2008 ; Vol. 294, No. 5.
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