Surgical denervation increases protein tyrosine phosphatase activity in skeletal muscle

Michael Tanowitz, Sun Hongwei, Lin Mei

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein tyrosine phosphorylation, which plays an important role in synapse formation at the neuromuscular junction, appears to be regulated by presynaptic neurons. Innervation increases whereas denervation decreases the phosphotyrosine content at the neuromuscular junction. The innervation-dependent tyrosine phosphorylation may result from elevated activity of protein tyrosine kinases; alternatively innervation may down-regulate the protein tyrosine phosphatase activity in the skeletal muscle. To investigate the possible neuronal control of protein tyrosine phosphatase activity at the neuromuscular junction, we have characterized protein tyrosine phosphatase activity in rat skeletal muscle and studied the effects of surgical denervation on the phosphatase activity. Protein tyrosine phosphatase activity in the skeletal muscle, assayed using src [32P]-phosphorylated myelin basic protein as a substrate, was both time- and protein concentration-dependent and was inhibited by micromolar concentrations of vanadate and zinc ion, both of which are known to inhibit tyrosine phosphatases specifically. It was not affected, however, by chemicals known to inhibit acid and alkaline phosphatases or serine/threonine phosphatases. Surgical denervation caused an increase in protein tyrosine phosphatase activity in rat hindlimb muscles. The increase in phosphatase activity reached a maximum (2-fold above the normal) 4 days post-denervation and maintained a plateau for up to 24 days. The biochemical properties of the phosphatase activity in denervated muscle were similar to those of the phosphatase activity in the innervated muscles. These results demonstrate that protein tyrosine phosphatase activity in skeletal muscle is regulated by motoneurons.

Original languageEnglish (US)
Pages (from-to)299-306
Number of pages8
JournalBrain Research
Volume712
Issue number2
DOIs
StatePublished - Mar 18 1996

Fingerprint

Protein Tyrosine Phosphatases
Denervation
Phosphoric Monoester Hydrolases
Skeletal Muscle
Neuromuscular Junction
Tyrosine
Muscles
Phosphorylation
Phosphotyrosine
Vanadates
Myelin Basic Protein
Phosphoprotein Phosphatases
Motor Neurons
Hindlimb
Acid Phosphatase
Protein-Tyrosine Kinases
Synapses
Alkaline Phosphatase
Zinc
Proteins

Keywords

  • denervation
  • neuromuscular
  • phosphatase
  • phosphorylation
  • synapse
  • synaptogenesis
  • tyrosine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Surgical denervation increases protein tyrosine phosphatase activity in skeletal muscle. / Tanowitz, Michael; Hongwei, Sun; Mei, Lin.

In: Brain Research, Vol. 712, No. 2, 18.03.1996, p. 299-306.

Research output: Contribution to journalArticle

Tanowitz, Michael ; Hongwei, Sun ; Mei, Lin. / Surgical denervation increases protein tyrosine phosphatase activity in skeletal muscle. In: Brain Research. 1996 ; Vol. 712, No. 2. pp. 299-306.
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